naa a22 4500 510730396 CHVBK 20180411082950.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s11295-013-0647-3 doi (NATIONALLICENCE)springer-10.1007/s11295-013-0647-3 Potentials and limitations of the cross-species transfer of nuclear microsatellite marker in six species belonging to three sections of the genus Populus L [Elektronische Daten] [Tobias Bruegmann, Matthias Fladung] The genus Populus is classified into six different sections, and depending on the declaration of hybrids, the number of species varies between 22 and 85. Species within one section, and sometimes between sections, are crossable to each other, resulting in many naturally but also artificially produced hybrids. Morphological attributes for a clone characterisation are often difficult to evaluate when different poplar species or even hybrids are crossed; thus, molecular markers are needed to characterise the different species. Taking advantage of the large microsatellite resource developed for Populus trichocarpa, however, amplification of these microsatellite markers in other Populus species either often fails, or in the case of amplification, unrelated genomic regions are amplified. To meet this obvious problem of the species transferability of microsatellite markers, in total, 305 microsatellite loci, mainly from P. trichocarpa but also few from Populus tremuloides and Populus nigra, were tested for their transferability to diverse genotypes of six species belonging to three sections of the genus Populus. Ultimately, 209 microsatellite loci could be amplified with varying sizes in the different species. The PCR products of selected loci were separated in a polyacrylamide gel and sequenced to assure that the expected loci were derived from the database genome of P. trichocarpa. The present results constitute a large study for microsatellite transferability for Populus species. The documented microsatellite loci can be applied to species-, hybrid- and clone-specific diagnostic approaches or as universal markers for comprehensive ecological studies. Springer-Verlag Berlin Heidelberg, 2013 Populus tremula nationallicence P. nigra nationallicence Cross-species amplification nationallicence DNA sequencing nationallicence Molecular marker nationallicence Poplar nationallicence Bruegmann Tobias Thuenen-Institute of Forest Genetics, Sieker Landstr. 2, 22927, Großhansdorf, Germany aut Fladung Matthias Thuenen-Institute of Forest Genetics, Sieker Landstr. 2, 22927, Großhansdorf, Germany aut Tree Genetics & Genomes Springer Berlin Heidelberg 9/6(2013-12-01), 1413-1421 1614-2942 9:6<1413 2013 9 11295 https://doi.org/10.1007/s11295-013-0647-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0647-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bruegmann Tobias Thuenen-Institute of Forest Genetics, Sieker Landstr. 2, 22927, Großhansdorf, Germany aut NATIONALLICENCE 700 1- Fladung Matthias Thuenen-Institute of Forest Genetics, Sieker Landstr. 2, 22927, Großhansdorf, Germany aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer Berlin Heidelberg 9/6(2013-12-01), 1413-1421 1614-2942 9:6<1413 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer