naa a22 4500 510730434 CHVBK 20180411082950.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s11295-013-0652-6 doi (NATIONALLICENCE)springer-10.1007/s11295-013-0652-6 Expression of DNA methyltransferases is involved in Quercus suber cork quality [Elektronische Daten] [Miguel Ramos, Margarida Rocheta, Luísa Carvalho, Vera Inácio, José Graça, Leonor Morais-Cecilio] Cork oak (Quercus suber) is an important Portuguese species, mainly due to the economic value of the cork it produces. Cork results from phellogen, a meristematic tissue, which can locally produce lenticels or have discontinuities, originating "defects”: pores and nail inclusions that are detrimental to cork industrial use. Epigenetic processes control plant development and its deregulation can lead to altered phenotypes; therefore, the study of epigenetic players in the phellogen is important to understand the emergence of cork's defects. DNA methyltransferases (DNMTs) and one protein associated to MET1 (DMAP1) were characterized in Q. suber, and their gene expression was analyzed in phellogen and contiguous differentiating cell layers of trees producing high and low quality cork, after the evaluation of their defects by physical and image analysis methods. All classes of DNMTs (MET, DRM, and CMT) with the respective canonical motifs were identified in Q. suber. The expression analyses of these genes showed that QsDRM2 was the most active methyltransferases in the cells analyzed, and that all the genes were differentially expressed in trees with distinct cork quality, with a tendency for higher expression levels in low quality producers. Interestingly, the global methylation level was higher in cells with low expression of DNA methyltransferases. A positive and significant correlation was obtained between QsDMAP1 gene expression and the percentage of cork defects. This work provides the first evidence that cork quality in Q. suber is likely influenced by epigenetic mechanisms. The Author(s), 2013 Cork oak nationallicence Cork quality nationallicence Phellogen activity nationallicence MET1 nationallicence MET2 nationallicence DRM nationallicence CMT3 nationallicence DMAP1 nationallicence Ramos Miguel Instituto Superior de Agronomia, CBAA, Universidade Técnica de Lisboa, Tapada da Ajuda, 1349-017, Lisbon, Portugal aut Rocheta Margarida Instituto Superior de Agronomia, CBAA, Universidade Técnica de Lisboa, Tapada da Ajuda, 1349-017, Lisbon, Portugal aut Carvalho Luísa Instituto Superior de Agronomia, CBAA, Universidade Técnica de Lisboa, Tapada da Ajuda, 1349-017, Lisbon, Portugal aut Inácio Vera Instituto Superior de Agronomia, CBAA, Universidade Técnica de Lisboa, Tapada da Ajuda, 1349-017, Lisbon, Portugal aut Graça José Instituto Superior de Agronomia, CEF, Universidade Técnica de Lisboa, Tapada da Ajuda, 1349-017, Lisbon, Portugal aut Morais-Cecilio Leonor Instituto Superior de Agronomia, CBAA, Universidade Técnica de Lisboa, Tapada da Ajuda, 1349-017, Lisbon, Portugal aut Tree Genetics & Genomes Springer Berlin Heidelberg 9/6(2013-12-01), 1481-1492 1614-2942 9:6<1481 2013 9 11295 https://doi.org/10.1007/s11295-013-0652-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0652-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ramos Miguel Instituto Superior de Agronomia, CBAA, Universidade Técnica de Lisboa, Tapada da Ajuda, 1349-017, Lisbon, Portugal aut NATIONALLICENCE 700 1- Rocheta Margarida Instituto Superior de Agronomia, CBAA, Universidade Técnica de Lisboa, Tapada da Ajuda, 1349-017, Lisbon, Portugal aut NATIONALLICENCE 700 1- Carvalho Luísa Instituto Superior de Agronomia, CBAA, Universidade Técnica de Lisboa, Tapada da Ajuda, 1349-017, Lisbon, Portugal aut NATIONALLICENCE 700 1- Inácio Vera Instituto Superior de Agronomia, CBAA, Universidade Técnica de Lisboa, Tapada da Ajuda, 1349-017, Lisbon, Portugal aut NATIONALLICENCE 700 1- Graça José Instituto Superior de Agronomia, CEF, Universidade Técnica de Lisboa, Tapada da Ajuda, 1349-017, Lisbon, Portugal aut NATIONALLICENCE 700 1- Morais-Cecilio Leonor Instituto Superior de Agronomia, CBAA, Universidade Técnica de Lisboa, Tapada da Ajuda, 1349-017, Lisbon, Portugal aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer Berlin Heidelberg 9/6(2013-12-01), 1481-1492 1614-2942 9:6<1481 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer