naa a22 4500 510730442 CHVBK 20180411082950.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s11295-013-0634-8 doi (NATIONALLICENCE)springer-10.1007/s11295-013-0634-8 Expression of the QrCPE gene is associated with the induction and development of oak somatic embryos [Elektronische Daten] [Silvia Valladares, Saleta Rico, Ana Vieitez, Purificación Covelo, Conchi Sánchez] Somatic embryogenesis is a powerful tool for plant regeneration and also provides a suitable material for investigating the molecular events that control the induction and development of somatic embryos. This study focuses on expression analysis of the QrCPE gene (which encodes a glycine-rich protein) during the initiation of oak somatic embryos from leaf explants and also during the histodifferentiation of somatic embryos. Northern blot and in situ hybridization were used to determine the specific localisation of QrCPE mRNA. The results showed that the QrCPE gene is developmentally regulated during the histodifferentiation of somatic embryos and that its expression is tissue- and genotype-dependent. QrCPE was strongly expressed in embryogenic cell aggregates and in embryogenic nodular structures originated in leaf explants as well as in the protodermis of somatic embryos from which new embryos are generated by secondary embryogenesis. This suggests a role for the gene during the induction of somatic embryos and in the maintenance of embryogenic competence. The QrCPE gene was highly expressed in actively dividing cells during embryo development, suggesting that it participates in embryo histodifferentiation. The localised expression in the root cap initial cells of cotyledonary somatic embryos and in the root cap of somatic seedlings also suggests that the gene may be involved in the fate of root cap cells. Springer-Verlag Berlin Heidelberg, 2013 Gene expression nationallicence Glycine-rich protein nationallicence Oak nationallicence QrCPE gene nationallicence Somatic embryogenesis nationallicence Valladares Silvia Department of Plant Physiology, Instituto de Investigaciones Agrobiológicas de Galicia (IIAG-CSIC), Apartado 122, 15780, Santiago de Compostela, Spain aut Rico Saleta Department of Plant Physiology, Instituto de Investigaciones Agrobiológicas de Galicia (IIAG-CSIC), Apartado 122, 15780, Santiago de Compostela, Spain aut Vieitez Ana Department of Plant Physiology, Instituto de Investigaciones Agrobiológicas de Galicia (IIAG-CSIC), Apartado 122, 15780, Santiago de Compostela, Spain aut Covelo Purificación Department of Plant Physiology, Instituto de Investigaciones Agrobiológicas de Galicia (IIAG-CSIC), Apartado 122, 15780, Santiago de Compostela, Spain aut Sánchez Conchi Department of Plant Physiology, Instituto de Investigaciones Agrobiológicas de Galicia (IIAG-CSIC), Apartado 122, 15780, Santiago de Compostela, Spain aut Tree Genetics & Genomes Springer Berlin Heidelberg 9/6(2013-12-01), 1383-1393 1614-2942 9:6<1383 2013 9 11295 https://doi.org/10.1007/s11295-013-0634-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0634-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Valladares Silvia Department of Plant Physiology, Instituto de Investigaciones Agrobiológicas de Galicia (IIAG-CSIC), Apartado 122, 15780, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Rico Saleta Department of Plant Physiology, Instituto de Investigaciones Agrobiológicas de Galicia (IIAG-CSIC), Apartado 122, 15780, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Vieitez Ana Department of Plant Physiology, Instituto de Investigaciones Agrobiológicas de Galicia (IIAG-CSIC), Apartado 122, 15780, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Covelo Purificación Department of Plant Physiology, Instituto de Investigaciones Agrobiológicas de Galicia (IIAG-CSIC), Apartado 122, 15780, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Sánchez Conchi Department of Plant Physiology, Instituto de Investigaciones Agrobiológicas de Galicia (IIAG-CSIC), Apartado 122, 15780, Santiago de Compostela, Spain aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer Berlin Heidelberg 9/6(2013-12-01), 1383-1393 1614-2942 9:6<1383 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer