naa a22 4500 510730450 CHVBK 20180411082950.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s11295-013-0650-8 doi (NATIONALLICENCE)springer-10.1007/s11295-013-0650-8 Signatures of adaptation and genetic structure among the mainland populations of Pinus radiata (D. Don) inferred from SNP loci [Elektronische Daten] [Shannon Dillon, Maureen Nolan, Philippe Matter, Washington Gapare, Jason Bragg, Simon Southerton] Insights into the relative contributions of locus specific and genome-wide effects on population genetic diversity can be gained through separation of their resulting genetic signals. Here we explore patterns of adaptive and neutral genetic diversity in the disjunct natural populations of Pinus radiata (D. Don) from mainland California. A first-generation common garden of 447 individuals revealed significant differentiation of wood phenotypes among populations (P ST), possibly reflecting local adaptation in response to environment. We subsequently screened all trees for genetic diversity at 149 candidate gene single nucleotide polymorphism (SNP) loci for signatures of adaptation. Ten loci were identified as being possible targets of diversifying selection following F ST outlier tests. Multivariate canonical correlation performed on a data set of 444 individuals identified significant covariance between environment, adaptive phenotypes and outlier SNP diversity, lending support to the case for local adaptation suggested from F ST and P ST tests. Covariation among discrete sets of outlier SNPs and adaptive phenotypes (inferred from multivariate loadings) with environment are supported by existing studies of candidate gene function and genotype-phenotype association. Canonical analyses failed to detect significant correlations between environment and 139 non-outlier SNP loci, which were applied to estimate neutral patterns of genetic differentiation among populations (F ST 4.3%). Using this data set, significant hierarchical structure was detected, indicating three populations on the mainland. The hierarchical relationships based on neutral SNP markers (and SSR) were in contrast with those inferred from putatively adaptive loci, potentially highlighting the independent action of selection and demography in shaping genetic structure in this species. Springer-Verlag Berlin Heidelberg, 2013 Radiata pine nationallicence SNP nationallicence Differentiation nationallicence Local adaptation nationallicence Population structure nationallicence Dillon Shannon CSIRO Plant Industry, PO Box E4008, 2604, Kingston, Australian Capital Territory, Australia aut Nolan Maureen CSIRO Plant Industry, PO Box E4008, 2604, Kingston, Australian Capital Territory, Australia aut Matter Philippe CSIRO Plant Industry, PO Box E4008, 2604, Kingston, Australian Capital Territory, Australia aut Gapare Washington CSIRO Plant Industry, PO Box E4008, 2604, Kingston, Australian Capital Territory, Australia aut Bragg Jason CSIRO Plant Industry, PO Box E4008, 2604, Kingston, Australian Capital Territory, Australia aut Southerton Simon CSIRO Plant Industry, PO Box E4008, 2604, Kingston, Australian Capital Territory, Australia aut Tree Genetics & Genomes Springer Berlin Heidelberg 9/6(2013-12-01), 1447-1463 1614-2942 9:6<1447 2013 9 11295 https://doi.org/10.1007/s11295-013-0650-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0650-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dillon Shannon CSIRO Plant Industry, PO Box E4008, 2604, Kingston, Australian Capital Territory, Australia aut NATIONALLICENCE 700 1- Nolan Maureen CSIRO Plant Industry, PO Box E4008, 2604, Kingston, Australian Capital Territory, Australia aut NATIONALLICENCE 700 1- Matter Philippe CSIRO Plant Industry, PO Box E4008, 2604, Kingston, Australian Capital Territory, Australia aut NATIONALLICENCE 700 1- Gapare Washington CSIRO Plant Industry, PO Box E4008, 2604, Kingston, Australian Capital Territory, Australia aut NATIONALLICENCE 700 1- Bragg Jason CSIRO Plant Industry, PO Box E4008, 2604, Kingston, Australian Capital Territory, Australia aut NATIONALLICENCE 700 1- Southerton Simon CSIRO Plant Industry, PO Box E4008, 2604, Kingston, Australian Capital Territory, Australia aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer Berlin Heidelberg 9/6(2013-12-01), 1447-1463 1614-2942 9:6<1447 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer