naa a22 4500 510730469 CHVBK 20180411082950.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s11295-013-0645-5 doi (NATIONALLICENCE)springer-10.1007/s11295-013-0645-5 Microsatellite-aided detection of genetic redundancy improves management of the International Cocoa Genebank, Trinidad [Elektronische Daten] [Lambert Motilal, Dapeng Zhang, Sue Mischke, Lyndel Meinhardt, Pathmanathan Umaharan] Cacao (Theobroma cacao L.), the tree from which cocoa butter and chocolate is derived, is conserved in field genebanks. The largest of these ex situ collections in the public domain is the International Cocoa Genebank, Trinidad (ICG,T). Reduction of genetic redundancy is essential to improve the accuracy and efficiency of genebank management. This study examined the pedigree and genetic diversity in a subset of 387 accessions in this collection. Sibship reconstruction of this subset revealed 56 full-sib families nested within 189 half-sib families. Sixteen centers of interconnectivity were identified, which suggested a high level of genetic redundancy in the collection. Generally, consistent phylogenetic trees were obtained using different genetic distance measures. However, a principal coordinate analysis of the D est differentiation measure elicited the best representation of accession group clustering, and we recommend this approach when probing fine-scale genetic differentiation among cacao accessions. The composite genetic diversity of 414 cacao accessions was contained in a core set of 59 unique accessions. These results have significant implications in the conservation of genetic resources of the ICG,T and other cacao genebanks. The approach developed in this study is recommended as a strategy to curators in guiding conservation management practices of cacao and other similar ex situ genebanks. Springer-Verlag Berlin Heidelberg, 2013 Cocoa nationallicence Conservation management nationallicence Sibship nationallicence Core collection nationallicence Field genebank nationallicence Redundancy nationallicence Motilal Lambert Cocoa Research Centre, The University of the West Indies, St. Augustine, Trinidad, Republic of Trinidad and Tobago aut Zhang Dapeng Beltsville Agricultural Research Center, SPCL, USDA/ARS, 10300 Baltimore Avenue, Bldg. 001, Rm. 223, BARC-W, 20705, Beltsville, MD, USA aut Mischke Sue Beltsville Agricultural Research Center, SPCL, USDA/ARS, 10300 Baltimore Avenue, Bldg. 001, Rm. 223, BARC-W, 20705, Beltsville, MD, USA aut Meinhardt Lyndel Beltsville Agricultural Research Center, SPCL, USDA/ARS, 10300 Baltimore Avenue, Bldg. 001, Rm. 223, BARC-W, 20705, Beltsville, MD, USA aut Umaharan Pathmanathan Cocoa Research Centre, The University of the West Indies, St. Augustine, Trinidad, Republic of Trinidad and Tobago aut Tree Genetics & Genomes Springer Berlin Heidelberg 9/6(2013-12-01), 1395-1411 1614-2942 9:6<1395 2013 9 11295 https://doi.org/10.1007/s11295-013-0645-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0645-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Motilal Lambert Cocoa Research Centre, The University of the West Indies, St. Augustine, Trinidad, Republic of Trinidad and Tobago aut NATIONALLICENCE 700 1- Zhang Dapeng Beltsville Agricultural Research Center, SPCL, USDA/ARS, 10300 Baltimore Avenue, Bldg. 001, Rm. 223, BARC-W, 20705, Beltsville, MD, USA aut NATIONALLICENCE 700 1- Mischke Sue Beltsville Agricultural Research Center, SPCL, USDA/ARS, 10300 Baltimore Avenue, Bldg. 001, Rm. 223, BARC-W, 20705, Beltsville, MD, USA aut NATIONALLICENCE 700 1- Meinhardt Lyndel Beltsville Agricultural Research Center, SPCL, USDA/ARS, 10300 Baltimore Avenue, Bldg. 001, Rm. 223, BARC-W, 20705, Beltsville, MD, USA aut NATIONALLICENCE 700 1- Umaharan Pathmanathan Cocoa Research Centre, The University of the West Indies, St. Augustine, Trinidad, Republic of Trinidad and Tobago aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer Berlin Heidelberg 9/6(2013-12-01), 1395-1411 1614-2942 9:6<1395 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer