naa a22 4500 510730485 CHVBK 20180411082950.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s11295-012-0584-6 doi (NATIONALLICENCE)springer-10.1007/s11295-012-0584-6 Sequencing of Sitka spruce ( Picea sitchensis ) cDNA libraries constructed from autumn buds and foliage reveals autumn-specific spruce transcripts [Elektronische Daten] [K. Reid, J. Holliday, M. Yuen, A. Nguyen, S. Aitken, J. Bohlmann] Substantial efforts have been invested in recent years to characterize the expressed genome of major North American spruce species, namely Sitka spruce (Picea sitchensis), white spruce (Picea glauca) and interior spruce (Picea engelmanii × glauca). To date, more than 550,000 spruce expressed sequence tags (ESTs) have been publically deposited, most of which were constructed from various tissue collected during active primary growth. Here we report EST sequencing of dormant foliage and bud tissue collected from Sitka spruce. Both normalized and standard libraries were employed, with tissue collected at two autumn time points. A total of 30,681 ESTs were generated then assembled into 9,400 putative unique transcripts, or unigenes, with an average length of 779bp. These autumn specific Sitka spruce ESTs were combined with autumn specific white spruce ESTs and compared with all spruce ESTs currently available. In total, 12,307 ESTs were unique to the autumn libraries, which assembled into 11,121 unigenes. Functional categorization suggests a role for some of these genes in bud dormancy and adaptation to freezing stress. Our results show that dormant tissue harbours a large number of transcripts not found in the same tissue during the growing season, and this sequence resource will therefore support ongoing studies of adaptive traits in spruce. Springer-Verlag Berlin Heidelberg, 2013 Expressed sequence tag nationallicence EST nationallicence Picea nationallicence Cold hardiness nationallicence Bud dormancy nationallicence Reid K. Michael Smith Laboratories, University of British Columbia, 2185 East Mall, V6T 1Z4, Vancouver, BC, Canada aut Holliday J. Department of Forest Sciences, University of British Columbia, 3041-2424 Main Mall, V6T 1Z4, Vancouver, BC, Canada aut Yuen M. Michael Smith Laboratories, University of British Columbia, 2185 East Mall, V6T 1Z4, Vancouver, BC, Canada aut Nguyen A. Michael Smith Laboratories, University of British Columbia, 2185 East Mall, V6T 1Z4, Vancouver, BC, Canada aut Aitken S. Department of Forest Sciences, University of British Columbia, 3041-2424 Main Mall, V6T 1Z4, Vancouver, BC, Canada aut Bohlmann J. Michael Smith Laboratories, University of British Columbia, 2185 East Mall, V6T 1Z4, Vancouver, BC, Canada aut Tree Genetics & Genomes Springer-Verlag 9/3(2013-06-01), 683-691 1614-2942 9:3<683 2013 9 11295 https://doi.org/10.1007/s11295-012-0584-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-012-0584-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Reid K. Michael Smith Laboratories, University of British Columbia, 2185 East Mall, V6T 1Z4, Vancouver, BC, Canada aut NATIONALLICENCE 700 1- Holliday J. Department of Forest Sciences, University of British Columbia, 3041-2424 Main Mall, V6T 1Z4, Vancouver, BC, Canada aut NATIONALLICENCE 700 1- Yuen M. Michael Smith Laboratories, University of British Columbia, 2185 East Mall, V6T 1Z4, Vancouver, BC, Canada aut NATIONALLICENCE 700 1- Nguyen A. Michael Smith Laboratories, University of British Columbia, 2185 East Mall, V6T 1Z4, Vancouver, BC, Canada aut NATIONALLICENCE 700 1- Aitken S. Department of Forest Sciences, University of British Columbia, 3041-2424 Main Mall, V6T 1Z4, Vancouver, BC, Canada aut NATIONALLICENCE 700 1- Bohlmann J. Michael Smith Laboratories, University of British Columbia, 2185 East Mall, V6T 1Z4, Vancouver, BC, Canada aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer-Verlag 9/3(2013-06-01), 683-691 1614-2942 9:3<683 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer