naa a22 4500 510730531 CHVBK 20180411082951.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s11295-013-0602-3 doi (NATIONALLICENCE)springer-10.1007/s11295-013-0602-3 Strength and variability of postmating reproductive isolating barriers between four European white oak species [Elektronische Daten] [O. Lepais, G. Roussel, F. Hubert, A. Kremer, S. Gerber] The identification and quantification of the relative importance of reproductive isolating barriers is of fundamental importance to understand species maintenance in the face of interspecific gene flow between hybridising species. Yet, such assessments require extensive experimental fertilisations that are particularly difficult when dealing with more than two hybridising and long-generation-time species such as oaks. Here, we quantify the relative contribution of four postmating reproductive isolating barriers consisting of two prezygotic barriers (gametic incompatibility, conspecific pollen precedence) and two postzygotic barriers (germination rate, early survival) from extensively controlled pollinations between four oak species (Quercus robur, Quercus petraea, Quercus pubescens and Quercus pyrenaica) that have been shown to frequently hybridise in natural populations. We found high variation in the strength of total reproductive isolation between species, ranging from total reproductive isolation to advantage toward hybrid formation. As previously found, Q. robur pollen was unable to fertilise Q. petraea due to a strong reproductive isolating mechanism. On the contrary, Q. pubescens pollen was more efficient at fertilising Q. petraea than conspecific pollen. Overall, prezygotic barriers contribute far more than postzygotic barriers to isolate species reproductively, suggesting a role for reinforcement in the development of prezygotic barriers. Conspecific pollen precedence reduced hybrid formation when pollen competition was allowed; however, presence of conspecific pollen did not totally prevent hybridization. Our results suggest that pollen competition depends on multiple ecological and environmental parameters, including species local abundance, and that it may be of uppermost importance to understand interspecific gene flow among natural multispecies populations. Springer-Verlag Berlin Heidelberg, 2013 Reproductive isolation nationallicence Controlled crosses nationallicence Pollen competition nationallicence Reinforcement nationallicence Hybridization nationallicence Quercus nationallicence Lepais O. UMR 1202 BIOGECO, INRA, 33610, Cestas, France aut Roussel G. UMR 1202 BIOGECO, INRA, 33610, Cestas, France aut Hubert F. UMR 1202 BIOGECO, INRA, 33610, Cestas, France aut Kremer A. UMR 1202 BIOGECO, INRA, 33610, Cestas, France aut Gerber S. UMR 1202 BIOGECO, INRA, 33610, Cestas, France aut Tree Genetics & Genomes Springer-Verlag 9/3(2013-06-01), 841-853 1614-2942 9:3<841 2013 9 11295 https://doi.org/10.1007/s11295-013-0602-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0602-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lepais O. UMR 1202 BIOGECO, INRA, 33610, Cestas, France aut NATIONALLICENCE 700 1- Roussel G. UMR 1202 BIOGECO, INRA, 33610, Cestas, France aut NATIONALLICENCE 700 1- Hubert F. UMR 1202 BIOGECO, INRA, 33610, Cestas, France aut NATIONALLICENCE 700 1- Kremer A. UMR 1202 BIOGECO, INRA, 33610, Cestas, France aut NATIONALLICENCE 700 1- Gerber S. UMR 1202 BIOGECO, INRA, 33610, Cestas, France aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer-Verlag 9/3(2013-06-01), 841-853 1614-2942 9:3<841 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer