naa a22 4500 510730574 CHVBK 20180411082951.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s11295-013-0598-8 doi (NATIONALLICENCE)springer-10.1007/s11295-013-0598-8 Characterization of genes associated with polyembryony and in vitro somatic embryogenesis in Citrus [Elektronische Daten] [Michiharu Nakano, Keiko Kigoshi, Tokurou Shimizu, Tomoko Endo, Takehiko Shimada, Hiroshi Fujii, Mitsuo Omura] Apomixis is a common reproduction system in the genus Citrus. To investigate the molecular mechanism of apomictic embryogenesis in Citrus, genes expressed specifically in an apomictic genotype were isolated by PCR-selected suppression subtractive hybridization with total RNAs obtained from the ovule at anthesis. Several genes showing conspicuously different expressions between polyembryonic (apomictic) and monoembryonic (nonapomictic) genotypes were selected, and their expression profiles during ovule development were analyzed in detail. This analysis identified two apomictic and three nonapomictic genotype-specific genes. Among the latter, msg-2 was highly expressed in the late stage of somatic embryogenesis. Specific expression during ovule development in monoembryonic cultivars and in the late stage of somatic embryogenesis indicated that msg-2 is not expressed in the initiation stage of polyembryogenesis and somatic embryogenesis, suggesting its role in suppressing initial cell formation of somatic embryos. The full-length complementary DNA of msg-2 contained small open reading frames in its sequence but showed no homology to functionally known genes in the public databases. As sequences similar to msg-2 were frequently found among Citrus expressed sequence tags, msg-2 may be associated with polyembryogenesis and somatic embryogenesis in a Citrus-specific manner. Springer-Verlag Berlin Heidelberg, 2013 Citrus nationallicence Apomixis nationallicence Suppression subtractive hybridization nationallicence Nucellus nationallicence Nakano Michiharu The United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, 501-1193, Gifu, Japan aut Kigoshi Keiko Faculty of Agriculture, Shizuoka University, 836 Ohya Suruga-ku, 422-8529, Shizuoka, Japan aut Shimizu Tokurou Department of Citrus Research, National Institute of Fruit Tree Science, 485-6 Okitsu-nakacho Shimizu-ku, 424-0292, Shizuoka, Japan aut Endo Tomoko Department of Citrus Research, National Institute of Fruit Tree Science, 485-6 Okitsu-nakacho Shimizu-ku, 424-0292, Shizuoka, Japan aut Shimada Takehiko Department of Citrus Research, National Institute of Fruit Tree Science, 485-6 Okitsu-nakacho Shimizu-ku, 424-0292, Shizuoka, Japan aut Fujii Hiroshi Department of Citrus Research, National Institute of Fruit Tree Science, 485-6 Okitsu-nakacho Shimizu-ku, 424-0292, Shizuoka, Japan aut Omura Mitsuo Faculty of Agriculture, Shizuoka University, 836 Ohya Suruga-ku, 422-8529, Shizuoka, Japan aut Tree Genetics & Genomes Springer-Verlag 9/3(2013-06-01), 795-803 1614-2942 9:3<795 2013 9 11295 https://doi.org/10.1007/s11295-013-0598-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0598-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakano Michiharu The United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, 501-1193, Gifu, Japan aut NATIONALLICENCE 700 1- Kigoshi Keiko Faculty of Agriculture, Shizuoka University, 836 Ohya Suruga-ku, 422-8529, Shizuoka, Japan aut NATIONALLICENCE 700 1- Shimizu Tokurou Department of Citrus Research, National Institute of Fruit Tree Science, 485-6 Okitsu-nakacho Shimizu-ku, 424-0292, Shizuoka, Japan aut NATIONALLICENCE 700 1- Endo Tomoko Department of Citrus Research, National Institute of Fruit Tree Science, 485-6 Okitsu-nakacho Shimizu-ku, 424-0292, Shizuoka, Japan aut NATIONALLICENCE 700 1- Shimada Takehiko Department of Citrus Research, National Institute of Fruit Tree Science, 485-6 Okitsu-nakacho Shimizu-ku, 424-0292, Shizuoka, Japan aut NATIONALLICENCE 700 1- Fujii Hiroshi Department of Citrus Research, National Institute of Fruit Tree Science, 485-6 Okitsu-nakacho Shimizu-ku, 424-0292, Shizuoka, Japan aut NATIONALLICENCE 700 1- Omura Mitsuo Faculty of Agriculture, Shizuoka University, 836 Ohya Suruga-ku, 422-8529, Shizuoka, Japan aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer-Verlag 9/3(2013-06-01), 795-803 1614-2942 9:3<795 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer