naa a22 4500 510730620 CHVBK 20180411082951.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s11295-012-0585-5 doi (NATIONALLICENCE)springer-10.1007/s11295-012-0585-5 Extensive selfing in an endangered population of Pinus parviflora var. parviflora (Pinaceae) in the Boso Hills, Japan [Elektronische Daten] [Takaya Iwasaki, Tadashi Sase, Shohei Takeda, Takeshi Ohsawa, Kemrio Ozaki, Naoki Tani, Hiroyuki Ikeda, Masanori Suzuki, Ryota Endo, Kazuo Tohei, Yasuyuki Watano] Wind-pollinated trees such as conifers have been considered relatively resistant to the deleterious effects of fragmentation and low density by virtue of their abundant production of airborne pollen. However, some studies indicated that population density affects the mating system even in wind-pollinated trees. In the present study, we examined the mating system and genetic structure of an endangered population (Boso) of Pinus parviflora var. parviflora, whose size has fallen by 80% in only the last quarter century. Genetic analyses using four nuclear microsatellite markers showed that the population was in Hardy-Weinberg equilibrium and harbored a high level of genetic variation comparable to that of a larger population. Additionally, only weak genetic structure was detected among local patches. On the other hand, the outcrossing rate of seeds in the Boso population was much lower (0.277 from MLTR and 0.297 from paternity analysis) than that found in a larger population of the species (0.778 from MLTR). The outcrossing rate for each mother tree was found to correlate positively with local density around the tree in the Boso population. This suggests that the extensive selfing would be a direct result of pollen limitation caused by the extremely low density of the population: an average of 0.201 trees per hectare for local patches. Because the adult population was in HWE, it is likely that the selfed seeds produced at present cannot contribute to population regeneration. The present study suggests that low population density would increase extinction risk even for wind-pollinated conifer populations. Springer-Verlag Berlin Heidelberg, 2013 Conifer nationallicence Conservation nationallicence Pinus parviflora var. parviflora nationallicence Pollen dispersal nationallicence Population density nationallicence Selfing nationallicence Iwasaki Takaya Department of General Systems Studies, Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, 153-8902, Tokyo, Japan aut Sase Tadashi Graduate School of Science, Chiba University, Inage-ku, 263-8522, Chiba, Japan aut Takeda Shohei Graduate School of Science, Chiba University, Inage-ku, 263-8522, Chiba, Japan aut Ohsawa Takeshi Graduate School of Science, Chiba University, Inage-ku, 263-8522, Chiba, Japan aut Ozaki Kemrio Natural History Museum and Institute, Chiba, Chuo-ku, 260-8682, Chiba, Japan aut Tani Naoki Forestry Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Ohwashi, 305-8686, Tsukuba, Japan aut Ikeda Hiroyuki The University of Tokyo Chiba Forest, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 299-5503, Kamogawa, Japan aut Suzuki Masanori The University of Tokyo Hokkaido Forest, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 079-1563, Furano, Japan aut Endo Ryota Forestry Research Institute, Chiba Prefectural Agriculture and Forestry Research Center, 289-1223, Sammu, Japan aut Tohei Kazuo Chiba Ecology Center, 292-0421, Kimitsu, Japan aut Watano Yasuyuki Graduate School of Science, Chiba University, Inage-ku, 263-8522, Chiba, Japan aut Tree Genetics & Genomes Springer-Verlag 9/3(2013-06-01), 693-705 1614-2942 9:3<693 2013 9 11295 https://doi.org/10.1007/s11295-012-0585-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-012-0585-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Iwasaki Takaya Department of General Systems Studies, Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, 153-8902, Tokyo, Japan aut NATIONALLICENCE 700 1- Sase Tadashi Graduate School of Science, Chiba University, Inage-ku, 263-8522, Chiba, Japan aut NATIONALLICENCE 700 1- Takeda Shohei Graduate School of Science, Chiba University, Inage-ku, 263-8522, Chiba, Japan aut NATIONALLICENCE 700 1- Ohsawa Takeshi Graduate School of Science, Chiba University, Inage-ku, 263-8522, Chiba, Japan aut NATIONALLICENCE 700 1- Ozaki Kemrio Natural History Museum and Institute, Chiba, Chuo-ku, 260-8682, Chiba, Japan aut NATIONALLICENCE 700 1- Tani Naoki Forestry Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Ohwashi, 305-8686, Tsukuba, Japan aut NATIONALLICENCE 700 1- Ikeda Hiroyuki The University of Tokyo Chiba Forest, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 299-5503, Kamogawa, Japan aut NATIONALLICENCE 700 1- Suzuki Masanori The University of Tokyo Hokkaido Forest, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 079-1563, Furano, Japan aut NATIONALLICENCE 700 1- Endo Ryota Forestry Research Institute, Chiba Prefectural Agriculture and Forestry Research Center, 289-1223, Sammu, Japan aut NATIONALLICENCE 700 1- Tohei Kazuo Chiba Ecology Center, 292-0421, Kimitsu, Japan aut NATIONALLICENCE 700 1- Watano Yasuyuki Graduate School of Science, Chiba University, Inage-ku, 263-8522, Chiba, Japan aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer-Verlag 9/3(2013-06-01), 693-705 1614-2942 9:3<693 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer