naa a22 4500 510730655 CHVBK 20180411082951.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s11295-013-0600-5 doi (NATIONALLICENCE)springer-10.1007/s11295-013-0600-5 Isolation and characterization of an AINTEGUMENTA -like gene in different coconut ( Cocos nucifera L.) varieties from Sri Lanka [Elektronische Daten] [H. Bandupriya, J. Gibbings, Jim Dunwell] Development of an efficient tissue culture protocol in coconut is hampered by numerous technical constraints. Thus a greater understanding of the fundamental aspects of embryogenesis is essential. The role of AINTEGUMENTA-like genes in embryogenesis has been elucidated not only in model plants but also in economically important crops. A coconut gene, CnANT, that encodes two APETALA2 (AP2) domains and a conserved linker region similar to those of the BABY BOOM transcription factor was cloned, characterized, and its tissue specific expression was examined. The full-length cDNA of 1,780bp contains a 1,425-bp open reading frame that encodes a putative peptide of 474 amino acids. The genomic DNA sequence includes 2,317bp and consists of nine exons interrupted by eight introns. The exon/intron organization of CnANT is similar to that of homologous genes in other plant species. Analysis of differential tissue expression by real-time polymerase chain reaction indicated that CnANT is expressed more highly in in vitro grown tissues than in other vegetative tissues. Sequence comparison of the genomic sequence of CnANT in different coconut varieties revealed one single nucleotide polymorphism and one indel in the first exon and first intron, respectively, which differentiate the Tall group of trees from Dwarfs. The indel sequence, which can be considered a simple sequence repeats marker, was successfully used to distinguish the Tall and Dwarf groups as well as to develop a marker system, which may be of value in the identification of parental varieties that are used in coconut breeding programs in Sri Lanka. Springer-Verlag Berlin Heidelberg, 2013 CnANT gene nationallicence Coconut varieties nationallicence Indel nationallicence Single nucleotide polymorphism nationallicence Bandupriya H. School of Biological Sciences, University of Reading, RG6 6AS, Reading, UK aut Gibbings J. School of Biological Sciences, University of Reading, RG6 6AS, Reading, UK aut Dunwell Jim School of Biological Sciences, University of Reading, RG6 6AS, Reading, UK aut Tree Genetics & Genomes Springer-Verlag 9/3(2013-06-01), 813-827 1614-2942 9:3<813 2013 9 11295 https://doi.org/10.1007/s11295-013-0600-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0600-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bandupriya H. School of Biological Sciences, University of Reading, RG6 6AS, Reading, UK aut NATIONALLICENCE 700 1- Gibbings J. School of Biological Sciences, University of Reading, RG6 6AS, Reading, UK aut NATIONALLICENCE 700 1- Dunwell Jim School of Biological Sciences, University of Reading, RG6 6AS, Reading, UK aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer-Verlag 9/3(2013-06-01), 813-827 1614-2942 9:3<813 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer