naa a22 4500 510730701 CHVBK 20180411082951.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s11295-013-0610-3 doi (NATIONALLICENCE)springer-10.1007/s11295-013-0610-3 A range wide geographic pattern of genetic diversity and population structure of Castanea mollissima populations inferred from nuclear and chloroplast microsatellites [Elektronische Daten] [Wei Liu, Ming Kang, Hua Tian, Hongwen Huang] Understanding geographical pattern of genetic diversity and population structure is of great importance for formulating conservation and utilization strategies. In this study, we investigated the genetic diversity and population structure of 28 natural populations of Castanea mollissima in China using eight nuclear and six chloroplast microsatellite makers (nSSRs and cpSSRs). Populations from central China harbored the highest genetic diversity at both nSSR and cpSSR markers (nSSR: H E = 0.705; cpSSR: H = 0.461). The standardized measure of genetic differentiation estimated as G′ ST was 0.447 for nSSR and 0.803 for cpSSR, respectively. The G′ST-based pollen to seed flow ratio is 3.043, indicating that pollen flow is not extensive among C. mollissima populations. No obvious population genetic structure by geographical locations was found by STRUCTURE analysis based on nSSR data, and similarly, no signal of phylogeographic structure was detected for cpSSR analysis. Five boundaries defining zones of maximum genetic differences within the network of the C. mollissima populations were found, and the locations of those barriers were consistent with those of four mountains, i.e., Daloushan Mountain, Dabashan Mountain, Wushan Mountain, and Qingliangfeng Mountain, indicating that those mountains might act as genetic barriers obstructing the genetic exchange among natural C. mollissima populations. These results provide valuable baseline data for conservation and utilization of this species. Springer-Verlag Berlin Heidelberg, 2013 Castanea mollissima nationallicence Microsatellite nationallicence Genetic diversity nationallicence Population structure nationallicence Liu Wei Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, Guangdong, China aut Kang Ming Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, Guangdong, China aut Tian Hua Wuhan Botanical Garden, Chinese Academy of Sciences, 430074, Wuhan, China aut Huang Hongwen Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, Guangdong, China aut Tree Genetics & Genomes Springer Berlin Heidelberg 9/4(2013-08-01), 975-987 1614-2942 9:4<975 2013 9 11295 https://doi.org/10.1007/s11295-013-0610-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0610-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Wei Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Kang Ming Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Tian Hua Wuhan Botanical Garden, Chinese Academy of Sciences, 430074, Wuhan, China aut NATIONALLICENCE 700 1- Huang Hongwen Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, Guangdong, China aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer Berlin Heidelberg 9/4(2013-08-01), 975-987 1614-2942 9:4<975 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer