naa a22 4500 510730728 CHVBK 20180411082951.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s11295-013-0611-2 doi (NATIONALLICENCE)springer-10.1007/s11295-013-0611-2 Diversity and cis -element architecture of the promoter regions of cellulose synthase genes in Eucalyptus [Elektronische Daten] [Nicky Creux, Minique De Castro, Martin Ranik, Mathabatha Maleka, Alexander Myburg] Lignocellulosic biomass from fast-growing plantation trees is composed of carbohydrate-rich materials deposited into plant cell walls in a coordinated manner during wood formation. The diversity and evolution of the transcriptional networks regulating this process have not been studied extensively. We investigated patterns of species-level nucleotide diversity in the promoters of cellulose synthase (CesA) genes from different Eucalyptus tree species and assessed the possible roles of DNA sequence polymorphism in the gain or loss of cis-elements harboured within the promoters. Promoter regions of three primary and three secondary cell wall-associated CesA genes were isolated from 13 Eucalyptus species and were analysed for nucleotide and cis-element diversity. Species-level nucleotide diversity (π) ranged from 0.014 to 0.068, and different CesA promoters exhibited distinct patterns of sequence conservation. A set of 22 putative cis-elements were mapped to the CesA promoters using in silico methods. Forty-two percent of the mapped cis-element occurrences contained singleton polymorphisms which resulted in either gain or loss of a cis-element in a particular Eucalyptus species. The promoters of Eucalyptus CesA genes contained regions that are highly conserved at the species (Eucalyptus) and genus (with Arabidopsis and Populus) level, suggesting the presence of regulatory modules imposing functional constraint on such regions. Nucleotide polymorphisms in the CesA promoters more frequently created new cis-element occurrences than disrupted existing cis-element occurrences, a process which may be important for the maintenance and evolution of cellulose gene regulation in plants. Springer-Verlag Berlin Heidelberg, 2013 cis -element conservation nationallicence Promoter evolution nationallicence Secondary cell wall nationallicence Wood formation nationallicence CesA nationallicence Woody biomass nationallicence Creux Nicky Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private Bag X20, 0028, Pretoria, South Africa aut De Castro Minique Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private Bag X20, 0028, Pretoria, South Africa aut Ranik Martin Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private Bag X20, 0028, Pretoria, South Africa aut Maleka Mathabatha Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private Bag X20, 0028, Pretoria, South Africa aut Myburg Alexander Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private Bag X20, 0028, Pretoria, South Africa aut Tree Genetics & Genomes Springer Berlin Heidelberg 9/4(2013-08-01), 989-1004 1614-2942 9:4<989 2013 9 11295 https://doi.org/10.1007/s11295-013-0611-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0611-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Creux Nicky Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private Bag X20, 0028, Pretoria, South Africa aut NATIONALLICENCE 700 1- De Castro Minique Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private Bag X20, 0028, Pretoria, South Africa aut NATIONALLICENCE 700 1- Ranik Martin Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private Bag X20, 0028, Pretoria, South Africa aut NATIONALLICENCE 700 1- Maleka Mathabatha Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private Bag X20, 0028, Pretoria, South Africa aut NATIONALLICENCE 700 1- Myburg Alexander Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private Bag X20, 0028, Pretoria, South Africa aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer Berlin Heidelberg 9/4(2013-08-01), 989-1004 1614-2942 9:4<989 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer