naa a22 4500 510730736 CHVBK 20180411082951.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s11295-013-0616-x doi (NATIONALLICENCE)springer-10.1007/s11295-013-0616-x Genetic investigations on the caffeine and chlorogenic acid relationship in an interspecific cross between Coffea liberica dewevrei and C. pseudozanguebariae [Elektronische Daten] [Chin-Long Ky, Philippe Barre, Michel Noirot] The objective of the paper was to identify the number of major loci explaining caffeine content in coffee seeds. Investigations were based on previously published results: (1) Caffeine binds to chlorogenic acids in a 1:1 molecular ratio; (2) Between species, the caffeine content is correlated to the chlorogenic acid content; (3) Only a part of chlorogenic acids is bound to caffeine. Especially, the content ratio between caffeine and chlorogenic acids varied between species. For identifying the number of major loci, a quantitative trait locus (QTL) approach was carried out using an interspecific cross between two highly differentiated species—Coffea liberica dewevrei and Coffea pseudozanguebariae, the latter being a caffeine-free species. As main finding, two QTLs, i.e., RCQ1 and CQA1, were identified allowing us to explain up to 97% of the caffeine content variance. RCQ1 explained variation of the caffeine/chlorogenic acid ratio and was genetically independent of the second QTL. The latter explained the part of the caffeine content which was dependent on the chlorogenic acid content. The findings also confirmed that only a part of chlorogenic acids were trapped by caffeine, as in wild species. Springer-Verlag Berlin Heidelberg, 2013 Coffea nationallicence Chlorogenic acids nationallicence Caffeine nationallicence QTL nationallicence Interspecific cross nationallicence Ky Chin-Long Centre IFREMER de Tahiti, Taravao, French Polynesia aut Barre Philippe Centre INRA de Lusignan, Lusignan, France aut Noirot Michel IRD, UMR PVBMT, Centre CIRAD Pôle de Protection des Plantes, 97410 St Pierre, La Réunion, France aut Tree Genetics & Genomes Springer Berlin Heidelberg 9/4(2013-08-01), 1043-1049 1614-2942 9:4<1043 2013 9 11295 https://doi.org/10.1007/s11295-013-0616-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0616-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ky Chin-Long Centre IFREMER de Tahiti, Taravao, French Polynesia aut NATIONALLICENCE 700 1- Barre Philippe Centre INRA de Lusignan, Lusignan, France aut NATIONALLICENCE 700 1- Noirot Michel IRD, UMR PVBMT, Centre CIRAD Pôle de Protection des Plantes, 97410 St Pierre, La Réunion, France aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer Berlin Heidelberg 9/4(2013-08-01), 1043-1049 1614-2942 9:4<1043 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer