naa a22 4500 510730744 CHVBK 20180411082951.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s11295-013-0615-y doi (NATIONALLICENCE)springer-10.1007/s11295-013-0615-y Transcriptome characterization and detection of gene expression differences in aspen ( Populus tremuloides ) [Elektronische Daten] [Hardeep Rai, Karen Mock, Bryce Richardson, Richard Cronn, Katherine Hayden, Jessica Wright, Brian Knaus, Paul Wolf] Aspen (Populus tremuloides) is a temperate North American tree species with a geographical distribution more extensive than any other tree species on the continent. Because it is economically important for pulp and paper industries and ecologically important for its role as a foundation species in forest ecosystems, the decline of aspen in large portions of its range is of serious concern. The availability and annotation of the black cottonwood (Populus trichocarpa) genome enables a range of high throughput sequencing approaches that can be used to understand rangewide patterns of genetic variation, adaptation, and responses to environmental challenges in other Populus species, including aspen. Gene expression studies are particularly useful for understanding the molecular basis of ecological responses, but are limited by the availability of transcriptome data. We explored the aspen transcriptome through the use of high-throughput sequencing with two main goals: (1) characterization of the expressed portion of the P. tremuloides genome in leaves and (2) assessment of variation in gene expression among genets collected from distinct latitudes but reared in a common garden. We also report a large single nucleotide polymorphism dataset that provides the groundwork for future studies of aspen evolution and ecology, and we identify a set of differentially expressed genes across individuals and population boundaries for the leaf transcriptome of P. tremuloides. Springer-Verlag Berlin Heidelberg (outside the USA), 2013 Trembling aspen nationallicence Quaking aspen nationallicence RNA-Seq nationallicence Differential expression nationallicence SNP (single nucleotide polymorphism) nationallicence Populus trichocarpa nationallicence Rai Hardeep Ecology Center and Department of Wildland Resources, Utah State University, 5230 Old Main Hill, 84322, Logan, UT, USA aut Mock Karen Ecology Center and Department of Wildland Resources, Utah State University, 5230 Old Main Hill, 84322, Logan, UT, USA aut Richardson Bryce Rocky Mountain Research Station, USDA Forest Service, 84606, Provo, UT, USA aut Cronn Richard Pacific Northwest Research Station, USDA Forest Service, 97331, Corvallis, OR, USA aut Hayden Katherine Department of Environmental Science, Policy, and Management, University of California, 94720, Berkeley, CA, USA aut Wright Jessica Pacific Southwest Research Station, USDA- Forest Service, 1731 Research Park Drive, 95618, Davis, CA, USA aut Knaus Brian Pacific Northwest Research Station, USDA Forest Service, 97331, Corvallis, OR, USA aut Wolf Paul Ecology Center and Department of Biology, Utah State University, 5305 Old Main Hill, 84322, Logan, UT, USA aut Tree Genetics & Genomes Springer Berlin Heidelberg 9/4(2013-08-01), 1031-1041 1614-2942 9:4<1031 2013 9 11295 https://doi.org/10.1007/s11295-013-0615-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0615-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rai Hardeep Ecology Center and Department of Wildland Resources, Utah State University, 5230 Old Main Hill, 84322, Logan, UT, USA aut NATIONALLICENCE 700 1- Mock Karen Ecology Center and Department of Wildland Resources, Utah State University, 5230 Old Main Hill, 84322, Logan, UT, USA aut NATIONALLICENCE 700 1- Richardson Bryce Rocky Mountain Research Station, USDA Forest Service, 84606, Provo, UT, USA aut NATIONALLICENCE 700 1- Cronn Richard Pacific Northwest Research Station, USDA Forest Service, 97331, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Hayden Katherine Department of Environmental Science, Policy, and Management, University of California, 94720, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Wright Jessica Pacific Southwest Research Station, USDA- Forest Service, 1731 Research Park Drive, 95618, Davis, CA, USA aut NATIONALLICENCE 700 1- Knaus Brian Pacific Northwest Research Station, USDA Forest Service, 97331, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Wolf Paul Ecology Center and Department of Biology, Utah State University, 5305 Old Main Hill, 84322, Logan, UT, USA aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer Berlin Heidelberg 9/4(2013-08-01), 1031-1041 1614-2942 9:4<1031 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer