naa a22 4500 510730760 CHVBK 20180411082951.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s11295-013-0618-8 doi (NATIONALLICENCE)springer-10.1007/s11295-013-0618-8 Class 1 non - symbiotic and class 3 truncated hemoglobin -like genes are differentially expressed in stone fruit rootstocks ( Prunus L.) with different degrees of tolerance to root hypoxia [Elektronische Daten] [Rubén Almada, María Arismendi, Paula Pimentel, Pamela Rojas, Patricio Hinrichsen, Manuel Pinto, Boris Sagredo] Root hypoxia produced by flooding or over-irrigation limits stone fruit tree development, particularly in orchards established on soils with restricted drainage. To overcome this problem, stone fruit trees are usually grafted on rootstocks (species or hybrid of the Prunus L. genus) with different degrees of tolerance to root hypoxia. However, the molecular base of such variability is largely unknown. In Arabidopsis thaliana (Heynh.), as well as in a number of crops and tree species, hemoglobin (Hb)-like genes stand out among hypoxia-related genes, but no such studies have been done with the Prunus species used as rootstocks. In this study, we analyzed the expression pattern of class 1 non-symbiotic Hb-like (nsHb) and class 3 truncated Hb-like (trHb) genes in Prunus rootstock roots with different responses to this stress. We observed that the putative Prunus nsHb and trHb genes were induced by root hypoxia in all analyzed Prunus genotypes, independently of their tolerance to hypoxia. However, Prunus nsHb and trHb genes had higher expression levels in roots of tolerant rootstocks. Prunus nsHb and trHb genes were also regulated by other abiotic stresses, such as salt stress and low temperatures. Our results suggest that changes in nsHb and trHb expressions could be part of the adaptive mechanisms that have evolved in the Prunus species to survive under hypoxia or other types of environmental stress that commonly challenge stone fruit tree orchards. Springer-Verlag Berlin Heidelberg, 2013 Hemoglobin nationallicence Prunus nationallicence Rootstocks nationallicence Root hypoxia nationallicence Waterlogging nationallicence Almada Rubén Centro de Estudios Avanzados en Fruticultura (CEAF), Instituto de Investigaciones Agropecuarias (INIA) CRI Rayentue, Av. Salamanca s/n, Sector Los Choapinos, Rengo, Chile aut Arismendi María Centro de Estudios Avanzados en Fruticultura (CEAF), Instituto de Investigaciones Agropecuarias (INIA) CRI Rayentue, Av. Salamanca s/n, Sector Los Choapinos, Rengo, Chile aut Pimentel Paula Centro de Estudios Avanzados en Fruticultura (CEAF), Instituto de Investigaciones Agropecuarias (INIA) CRI Rayentue, Av. Salamanca s/n, Sector Los Choapinos, Rengo, Chile aut Rojas Pamela Centro de Estudios Avanzados en Fruticultura (CEAF), Instituto de Investigaciones Agropecuarias (INIA) CRI Rayentue, Av. Salamanca s/n, Sector Los Choapinos, Rengo, Chile aut Hinrichsen Patricio Centro de Estudios Avanzados en Fruticultura (CEAF), Instituto de Investigaciones Agropecuarias (INIA) CRI Rayentue, Av. Salamanca s/n, Sector Los Choapinos, Rengo, Chile aut Pinto Manuel Centro de Estudios Avanzados en Fruticultura (CEAF), Instituto de Investigaciones Agropecuarias (INIA) CRI Rayentue, Av. Salamanca s/n, Sector Los Choapinos, Rengo, Chile aut Sagredo Boris Centro de Estudios Avanzados en Fruticultura (CEAF), Instituto de Investigaciones Agropecuarias (INIA) CRI Rayentue, Av. Salamanca s/n, Sector Los Choapinos, Rengo, Chile aut Tree Genetics & Genomes Springer Berlin Heidelberg 9/4(2013-08-01), 1051-1063 1614-2942 9:4<1051 2013 9 11295 https://doi.org/10.1007/s11295-013-0618-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0618-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Almada Rubén Centro de Estudios Avanzados en Fruticultura (CEAF), Instituto de Investigaciones Agropecuarias (INIA) CRI Rayentue, Av. Salamanca s/n, Sector Los Choapinos, Rengo, Chile aut NATIONALLICENCE 700 1- Arismendi María Centro de Estudios Avanzados en Fruticultura (CEAF), Instituto de Investigaciones Agropecuarias (INIA) CRI Rayentue, Av. Salamanca s/n, Sector Los Choapinos, Rengo, Chile aut NATIONALLICENCE 700 1- Pimentel Paula Centro de Estudios Avanzados en Fruticultura (CEAF), Instituto de Investigaciones Agropecuarias (INIA) CRI Rayentue, Av. Salamanca s/n, Sector Los Choapinos, Rengo, Chile aut NATIONALLICENCE 700 1- Rojas Pamela Centro de Estudios Avanzados en Fruticultura (CEAF), Instituto de Investigaciones Agropecuarias (INIA) CRI Rayentue, Av. Salamanca s/n, Sector Los Choapinos, Rengo, Chile aut NATIONALLICENCE 700 1- Hinrichsen Patricio Centro de Estudios Avanzados en Fruticultura (CEAF), Instituto de Investigaciones Agropecuarias (INIA) CRI Rayentue, Av. Salamanca s/n, Sector Los Choapinos, Rengo, Chile aut NATIONALLICENCE 700 1- Pinto Manuel Centro de Estudios Avanzados en Fruticultura (CEAF), Instituto de Investigaciones Agropecuarias (INIA) CRI Rayentue, Av. Salamanca s/n, Sector Los Choapinos, Rengo, Chile aut NATIONALLICENCE 700 1- Sagredo Boris Centro de Estudios Avanzados en Fruticultura (CEAF), Instituto de Investigaciones Agropecuarias (INIA) CRI Rayentue, Av. Salamanca s/n, Sector Los Choapinos, Rengo, Chile aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer Berlin Heidelberg 9/4(2013-08-01), 1051-1063 1614-2942 9:4<1051 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer