naa a22 4500 510730779 CHVBK 20180411082951.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s11295-013-0612-1 doi (NATIONALLICENCE)springer-10.1007/s11295-013-0612-1 Asymmetric introgression between Magnolia stellata and M [Elektronische Daten] salicifolia at a site where the two species grow sympatrically [Shuhei Muranishi, Ichiro Tamaki, Suzuki Setsuko, Nobuhiro Tomaru] In order to understand the ongoing evolutionary relationships between species, it is important to elucidate patterns of natural hybridization. In the zone where two species are sympatrically distributed, we examined 274 individuals of Magnolia stellata, Magnolia salicifolia, and their putative hybrids by means of 16 nuclear and three chloroplast microsatellite markers. Hybrid classes of individuals were estimated by admixture analyses. Morphological traits were also investigated for 64 of the 274 individuals. Admixture analyses revealed that 66 of the 274 individuals were classified as hybrids, comprising 17 F1 and 19 F2 individuals, 27 backcrosses to M. salicifolia, and 3 individuals of unknown origin. Morphological data from the 64 individuals agreed well with their genetic admixture rates. Spatial locations of F1 and F2 hybrids at the study site were intermediate between the two purebred species, indicating that the site preferences of hybrids are intermediate. The occurrences of F2 and backcross hybrids indicate that F1 hybrids are fertile. The chloroplast DNA haplotypes of all F1 hybrids corresponded to those detected in M. salicifolia, so that maternal parents of the F1 hybrids were all M. salicifolia. Furthermore, no hybrid individuals derived from a backcross to M. stellata were detected. These results suggest that the direction of hybridization and the subsequent introgression have been quite asymmetric and that the introgression occurred from M. stellata into M. salicifolia. Springer-Verlag Berlin Heidelberg, 2013 Backcross nationallicence F1 nationallicence F2 nationallicence Hybridization nationallicence Magnoliaceae nationallicence Microsatellite nationallicence Site preferences of hybrids nationallicence Muranishi Shuhei Graduate School of Bioagricultural and Sciences, Nagoya University, 464-8601, Nagoya, Japan aut Tamaki Ichiro Gifu Academy of Forest Science and Culture, 88 Sodai, 501-3714, Mino, Gifu, Japan aut Setsuko Suzuki Forestry and Forest Products Research Institute, 305-8687, Tsukuba, Ibaraki, Japan aut Tomaru Nobuhiro Graduate School of Bioagricultural and Sciences, Nagoya University, 464-8601, Nagoya, Japan aut Tree Genetics & Genomes Springer Berlin Heidelberg 9/4(2013-08-01), 1005-1015 1614-2942 9:4<1005 2013 9 11295 https://doi.org/10.1007/s11295-013-0612-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0612-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Muranishi Shuhei Graduate School of Bioagricultural and Sciences, Nagoya University, 464-8601, Nagoya, Japan aut NATIONALLICENCE 700 1- Tamaki Ichiro Gifu Academy of Forest Science and Culture, 88 Sodai, 501-3714, Mino, Gifu, Japan aut NATIONALLICENCE 700 1- Setsuko Suzuki Forestry and Forest Products Research Institute, 305-8687, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Tomaru Nobuhiro Graduate School of Bioagricultural and Sciences, Nagoya University, 464-8601, Nagoya, Japan aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer Berlin Heidelberg 9/4(2013-08-01), 1005-1015 1614-2942 9:4<1005 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer