naa a22 4500 510730876 CHVBK 20180411082952.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s11295-013-0622-z doi (NATIONALLICENCE)springer-10.1007/s11295-013-0622-z De novo sequencing and comparative analysis of expressed sequence tags from gynodioecious fig ( Ficus carica L.) fruits: caprifig and common fig [Elektronische Daten] [Hidetoshi Ikegami, Tsuyoshi Habu, Kazuki Mori, Hitoshi Nogata, Chiharu Hirata, Keita Hirashima, Kousuke Tashiro, Satoru Kuhara] We conducted an exhaustive study of gene expression in fig fruits to identify the gene complexes responsible for fundamental fruit physiology and phenotypic differences between ecotypes. We performed high-throughput pyrosequencing on cDNA libraries constructed from caprifig and common fig fruits and compared their transcriptomes by analyzing the expressed sequence tags obtained. We collected a total of 290,594 expressed sequence tag reads from the two fruit types and assembled them into 71,455 unigenes (19,166 contigs and 52,289 singletons). We identified many metabolic genes, including those encoding proteins in the ethylene, glucose, and anthocyanin synthesis pathways that are involved in fruit maturation. This set also contained unigenes with unidentified functions. We observed no significant differences between the fruit types with respect to Gene Ontology term representation. By reverse transcription polymerase chain reaction, however, we detected several polymorphisms at the level of individual genes. Inter-type variations with respect to the expression level or transcription product size were observed in B- and C-class MADS-box gene homologs and chalcone synthase homologs, which are believed to be involved in sexuality and parthenocarpy, respectively. Expression polymorphisms were also observed for other genes, including a gibberellin-regulated protein gene. Our data and results contribute to genetic research on fig fruits and will aid in the understanding of fruit physiology and mechanisms of phenotypic differentiation. Springer-Verlag Berlin Heidelberg, 2013 Ficus carica nationallicence Caprifig nationallicence Common fig nationallicence Gynodioecious nationallicence Expressed sequence tag nationallicence Ikegami Hidetoshi Fukuoka Agricultural Research Center, 587 Yoshiki, 818-8549, Chikushino, Fukuoka, Japan aut Habu Tsuyoshi Faculty of Agriculture, Ehime University, 790-8566, Matsuyama, Japan aut Mori Kazuki Faculty of Agriculture, Kyushu University, 812-8581, Fukuoka, Japan aut Nogata Hitoshi Fukuoka Control Station for Pests, 423 Yoshiki, 818-0004, Chikushino, Fukuoka, Japan aut Hirata Chiharu Fukuoka Agricultural Research Center, 587 Yoshiki, 818-8549, Chikushino, Fukuoka, Japan aut Hirashima Keita Fukuoka Agricultural Research Center, 587 Yoshiki, 818-8549, Chikushino, Fukuoka, Japan aut Tashiro Kousuke Faculty of Agriculture, Kyushu University, 812-8581, Fukuoka, Japan aut Kuhara Satoru Faculty of Agriculture, Kyushu University, 812-8581, Fukuoka, Japan aut Tree Genetics & Genomes Springer Berlin Heidelberg 9/4(2013-08-01), 1075-1088 1614-2942 9:4<1075 2013 9 11295 https://doi.org/10.1007/s11295-013-0622-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0622-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ikegami Hidetoshi Fukuoka Agricultural Research Center, 587 Yoshiki, 818-8549, Chikushino, Fukuoka, Japan aut NATIONALLICENCE 700 1- Habu Tsuyoshi Faculty of Agriculture, Ehime University, 790-8566, Matsuyama, Japan aut NATIONALLICENCE 700 1- Mori Kazuki Faculty of Agriculture, Kyushu University, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 700 1- Nogata Hitoshi Fukuoka Control Station for Pests, 423 Yoshiki, 818-0004, Chikushino, Fukuoka, Japan aut NATIONALLICENCE 700 1- Hirata Chiharu Fukuoka Agricultural Research Center, 587 Yoshiki, 818-8549, Chikushino, Fukuoka, Japan aut NATIONALLICENCE 700 1- Hirashima Keita Fukuoka Agricultural Research Center, 587 Yoshiki, 818-8549, Chikushino, Fukuoka, Japan aut NATIONALLICENCE 700 1- Tashiro Kousuke Faculty of Agriculture, Kyushu University, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 700 1- Kuhara Satoru Faculty of Agriculture, Kyushu University, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer Berlin Heidelberg 9/4(2013-08-01), 1075-1088 1614-2942 9:4<1075 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer