naa a22 4500 510730884 CHVBK 20180411082952.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s11295-013-0628-6 doi (NATIONALLICENCE)springer-10.1007/s11295-013-0628-6 Can gene flow among populations counteract the habitat loss of extremely fragile biotopes? An example from the population genetic structure in Salix daphnoides [Elektronische Daten] [Michal Sochor, Radim Vašut, Eva Bártová, Ľuboš Majeský, Jaroslav Mráček] Wild river gravel banks (RGB) represent an extremely fragile biotope that is significantly endangered by human activities due to its fragmentation over the past century. The consequences of such processes were studied using the endangered violet willow (Salix daphnoides) at the westernmost foothills of the Carpathian Mountains. We quantified population genetic characteristics for 14 ecologically and demographically characterised populations using simple sequence repeats (SSR) and amplified fragment length polymorphism (AFLP) markers. We found a significant correlation between the biotope, sex, age and genetic structure of the populations. The natural RGB populations revealed high genotypic variability when using SSR markers, in contrast to low genotypic variability of the populations of other biotopes of Cirsium wet meadows (CWM) and Ash-alder forests (AAF) that consisted of one to two clones at each site. High heterozygosity (H obs = 0.428-0.532) was similar across all natural (RGB) populations; however, these populations were deficient in heterozygotes (F IS/ρ IS > 0). All RGB populations exhibited moderate to very significant genetic differentiation for microsatellites, despite the fact that the AFLP data showed strong differentiation only between CWM and AAF populations. Division into clusters by Structure confirmed consistent geographic groups for the RGB populations. According to our results, the strong decrease in previously continuous and large natural habitats for the violet willow is slightly counterbalanced by among-population gene flow. However, the survival of natural populations is tightly linked to the presence of river gravel banks and bars. Strict protection of this habitat is therefore essential for the conservation of the species. Springer-Verlag Berlin Heidelberg, 2013 Conservation nationallicence Genetic diversity nationallicence Population genetics nationallicence River gravel banks nationallicence Salix daphnoides nationallicence Willow nationallicence Sochor Michal Department of Botany, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 11, 78371, Olomouc, Czech Republic aut Vašut Radim Department of Botany, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 11, 78371, Olomouc, Czech Republic aut Bártová Eva Department of Botany, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 11, 78371, Olomouc, Czech Republic aut Majeský Ľuboš Department of Botany, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 11, 78371, Olomouc, Czech Republic aut Mráček Jaroslav Department of Forest Botany, Dendrology and Geobiocoenology, Mendel University in Brno, Zemědělská 3, 61300, Brno, Czech Republic aut Tree Genetics & Genomes Springer Berlin Heidelberg 9/5(2013-10-01), 1193-1205 1614-2942 9:5<1193 2013 9 11295 https://doi.org/10.1007/s11295-013-0628-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0628-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sochor Michal Department of Botany, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 11, 78371, Olomouc, Czech Republic aut NATIONALLICENCE 700 1- Vašut Radim Department of Botany, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 11, 78371, Olomouc, Czech Republic aut NATIONALLICENCE 700 1- Bártová Eva Department of Botany, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 11, 78371, Olomouc, Czech Republic aut NATIONALLICENCE 700 1- Majeský Ľuboš Department of Botany, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 11, 78371, Olomouc, Czech Republic aut NATIONALLICENCE 700 1- Mráček Jaroslav Department of Forest Botany, Dendrology and Geobiocoenology, Mendel University in Brno, Zemědělská 3, 61300, Brno, Czech Republic aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer Berlin Heidelberg 9/5(2013-10-01), 1193-1205 1614-2942 9:5<1193 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer