naa a22 4500 510730965 CHVBK 20180411082952.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s11295-013-0644-6 doi (NATIONALLICENCE)springer-10.1007/s11295-013-0644-6 Molecular cloning and gene expression differences of the anthocyanin biosynthesis-related genes in the red/green skin color mutant of pear ( Pyrus communis L.) [Elektronische Daten] [Ya-nan Yang, Guang Zhao, Wen-quan Yue, Shao-ling Zhang, Chao Gu, Jun Wu] To reveal the molecular mechanisms that led to the red/green color mutation of pear between the cultivar ‘Early red Doyenne du Comice' and its green variant strain, the full-length cDNA of the seven anthocyanin biosynthesis genes (PAL, CHS, CHI, DFR, F3H, ANS, UFGT) was cloned in both cultivars. The accession number has been submitted to National Center for Biotechnology Information (NCBI) as KC460392, KC460393, KC460394, KC460395, KC460396, KC460397, and KC460398, respectively. However, there was no sequence difference between the color mutants, which means that the skin color change was not caused by mutation of any of these genes. Meanwhile, the expression levels of these seven genes were examined by quantitative real-time PCR (qRT-PCR). Results showed that most of the structural genes were up-regulated in the red-skinned cultivar during fruit development, but the CHI and UFGT genes were highly expressed only at an early stage. The expression levels of the transcription factors MYB10, bHLH, and WD40 were also investigated by qRT-PCR, and the MYB10 gene was found to be expressed at significantly higher levels in the red variety than in the green mutant at the early stage, while the expression levels of bHLH and WD40 were higher at a later stage. These data indicate that the expression difference of structural genes in the anthocyanin biosynthesis pathway led to the skin color change of the mutant. However, MYB10, bHLH, and WD40 do not appear to be the key transcription factors that regulate the biosynthesis of anthocyanin and determine the red/green color mutant. Springer-Verlag Berlin Heidelberg, 2013 Pear nationallicence Color mutant nationallicence Anthocyanin nationallicence Molecular cloning nationallicence Gene expression nationallicence ANS : Anthocyanin synthase nationallicence bHLH : Basic helix loop helix nationallicence CHI : Chalcone isomerase nationallicence CHS : Chalcone synthase nationallicence DAFB : Days after full bloom nationallicence DFR : Dihydroflavonol-4-reductase nationallicence F3H : Flavanone 3-hydroxylase nationallicence PAL : Phenylalanine ammonialyase nationallicence RACE : Rapid amplification of cDNA ends nationallicence qRT-PCR : Quantitative reverse transcription polymerase chain reaction nationallicence SE : Standard error nationallicence TAIL-PCR : Thermal asymmetric interlaced polymerase chain reaction nationallicence UFGT : UDP-glucose: flavonoid-3-O-glucosyltransferase nationallicence Yang Ya-nan Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut Zhao Guang Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut Yue Wen-quan Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, 066600, Changli, Hebei, People's Republic of China aut Zhang Shao-ling Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut Gu Chao Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut Wu Jun Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut Tree Genetics & Genomes Springer Berlin Heidelberg 9/5(2013-10-01), 1351-1360 1614-2942 9:5<1351 2013 9 11295 https://doi.org/10.1007/s11295-013-0644-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0644-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Ya-nan Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Guang Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Yue Wen-quan Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, 066600, Changli, Hebei, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Shao-ling Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Gu Chao Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Wu Jun Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer Berlin Heidelberg 9/5(2013-10-01), 1351-1360 1614-2942 9:5<1351 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer