naa a22 4500 510730973 CHVBK 20180411082952.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s11295-013-0632-x doi (NATIONALLICENCE)springer-10.1007/s11295-013-0632-x Epigenetic characterization of chromatin in cycling cells of pedunculate oak, Quercus robur L [Elektronische Daten] [V. Vičić, D. Barišić, T. Horvat, I. Biruš, Vlatka Zoldos] Cycling cells of Quercus robur have a simple nuclear organization where most of the heterochromatin is visible as DAPI-positive chromocenters, which correspond to DAPI bands at the (peri)centromeric region of each of the 24 chromosomes of the oak complement. Immunofluorescence using 5-mC revealed dispersed distribution of the signal throughout the interphase nucleus/chromosomes without enrichment within DAPI-positive chromocenters/bands, suggesting that DNA methylation was not restricted to constitutive heterochromatin, but was associated with both euchromatic and heterochromatic domains. While H3K9ac exhibited typical euchromatin-specific distribution, the distributional pattern of histone methylation marks H3K9me1, H3K27me2, and H3K4me3 showed some specificity. The H3K9me1 and H3K27me2, both heterochromatin-associated marks, were not restricted to chromocenters, but showed additional dispersed distribution within euchromatin, while H3K27me2 mark also clustered in foci that did not co-localize with chromocenters. Surprisingly, even though H3K4me3 was distributed in the entire chromatin, many chromocenters were enriched with this euchromatin-specific modification. We discuss the distribution of the epigenetic marks in the context of the genome composition and lifestyle of Q. robur. Springer-Verlag Berlin Heidelberg, 2013 Interphase chromatin nationallicence Chromocenters nationallicence DNA methylation nationallicence Epigenetics nationallicence Histone acetylation nationallicence Histone methylation nationallicence Quercus robur nationallicence Vičić V. Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10 000, Zagreb, Croatia aut Barišić D. Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10 000, Zagreb, Croatia aut Horvat T. Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10 000, Zagreb, Croatia aut Biruš I. Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10 000, Zagreb, Croatia aut Zoldos Vlatka Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10 000, Zagreb, Croatia aut Tree Genetics & Genomes Springer Berlin Heidelberg 9/5(2013-10-01), 1247-1256 1614-2942 9:5<1247 2013 9 11295 https://doi.org/10.1007/s11295-013-0632-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0632-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vičić V. Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10 000, Zagreb, Croatia aut NATIONALLICENCE 700 1- Barišić D. Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10 000, Zagreb, Croatia aut NATIONALLICENCE 700 1- Horvat T. Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10 000, Zagreb, Croatia aut NATIONALLICENCE 700 1- Biruš I. Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10 000, Zagreb, Croatia aut NATIONALLICENCE 700 1- Zoldos Vlatka Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10 000, Zagreb, Croatia aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer Berlin Heidelberg 9/5(2013-10-01), 1247-1256 1614-2942 9:5<1247 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer