naa a22 4500 510730981 CHVBK 20180411082952.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s11295-013-0638-4 doi (NATIONALLICENCE)springer-10.1007/s11295-013-0638-4 High genetic diversity of in situ and ex situ populations of Madagascan coffee species: further implications for the management of coffee genetic resources [Elektronische Daten] [Domohina Andrianasolo, Aaron Davis, Norosoa Razafinarivo, Serge Hamon, Jean-Jacques Rakotomalala, Sylvie-Annabel Sabatier, Perla Hamon] The genetic diversity of endemic Madagascan Coffea species has not received any considerable attention, despite the high extinction threat facing most of the species on the island. In order to address this shortfall, we assessed and compared the genetic diversity and allelic richness of ex situ and in situ populations for four selected species, and 18 ex situ populations maintained at the Kianjavato Coffee Research Station. We also investigated the possibility of in situ and ex situ hybridization events for Madagascan coffee, both within and between species. For these purposes, we used 18 nuclear microsatellite markers, on a total of 37 Coffea populations (4 in situ and 33 ex situ) representing a total of 398 genotypes (96 in situ and 302 ex situ) for 23 species. Our study showed (1) strong differentiation between Madagascan Coffea species; (2) good differentiation between all the populations studied, including those from the same locality (even when the area of the locality was restricted); (3) good evidence for both in situ and ex situ hybridization, although in situ hybridization appeared to be limited; (4) much higher genetic diversity within in situ collections compared to ex situ collections; and (5) that, despite limitations, the Kianjavato Coffee Research Station collection is an extremely valuable resource of Madagascan coffee germplasm. Recommendations for in situ and ex situ germplasm conservation are discussed. Springer-Verlag Berlin Heidelberg, 2013 Coffea nationallicence Genetic resources nationallicence Genetic diversity nationallicence Madagascar nationallicence Microsatellite markers nationallicence Conservation nationallicence EOO : Extent of occurrence nationallicence FAO : Food and agriculture organization nationallicence FOFIFA : Foibe fikarohana ampiharina ho fampandrosoana ny eny ambanivohitra nationallicence ICO : International coffee organization nationallicence IUCN : International union for conservation of nature nationallicence KCRS : Kianjavato coffee research station nationallicence PCA : Principal component analysis nationallicence SSRs : Simple sequence repeats nationallicence Andrianasolo Domohina UMR AMAP, CIRAD, Boulevard de la Lironde TA A-51/PS2, F-34398 Cedex 5, Montpellier, France aut Davis Aaron Royal Botanic Gardens, Kew, TW9 3AB, Richmond, Surrey, UK aut Razafinarivo Norosoa UMR DIADE, IRD, B.P. 64501, 34394 Cedex 5, Montpellier, France aut Hamon Serge UMR DIADE, IRD, B.P. 64501, 34394 Cedex 5, Montpellier, France aut Rakotomalala Jean-Jacques FOFIFA, B.P. 1444, Ambatobe, Antananarivo 101, Madagascar aut Sabatier Sylvie-Annabel UMR AMAP, CIRAD, Boulevard de la Lironde TA A-51/PS2, F-34398 Cedex 5, Montpellier, France aut Hamon Perla UMR DIADE, IRD, B.P. 64501, 34394 Cedex 5, Montpellier, France aut Tree Genetics & Genomes Springer Berlin Heidelberg 9/5(2013-10-01), 1295-1312 1614-2942 9:5<1295 2013 9 11295 https://doi.org/10.1007/s11295-013-0638-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0638-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Andrianasolo Domohina UMR AMAP, CIRAD, Boulevard de la Lironde TA A-51/PS2, F-34398 Cedex 5, Montpellier, France aut NATIONALLICENCE 700 1- Davis Aaron Royal Botanic Gardens, Kew, TW9 3AB, Richmond, Surrey, UK aut NATIONALLICENCE 700 1- Razafinarivo Norosoa UMR DIADE, IRD, B.P. 64501, 34394 Cedex 5, Montpellier, France aut NATIONALLICENCE 700 1- Hamon Serge UMR DIADE, IRD, B.P. 64501, 34394 Cedex 5, Montpellier, France aut NATIONALLICENCE 700 1- Rakotomalala Jean-Jacques FOFIFA, B.P. 1444, Ambatobe, Antananarivo 101, Madagascar aut NATIONALLICENCE 700 1- Sabatier Sylvie-Annabel UMR AMAP, CIRAD, Boulevard de la Lironde TA A-51/PS2, F-34398 Cedex 5, Montpellier, France aut NATIONALLICENCE 700 1- Hamon Perla UMR DIADE, IRD, B.P. 64501, 34394 Cedex 5, Montpellier, France aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer Berlin Heidelberg 9/5(2013-10-01), 1295-1312 1614-2942 9:5<1295 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer