naa a22 4500 510731007 CHVBK 20180411082952.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s11295-013-0643-7 doi (NATIONALLICENCE)springer-10.1007/s11295-013-0643-7 The effects of artificial selection on sugar metabolism and transporter genes in grape [Elektronische Daten] [Haiping Xin, Jisen Zhang, Wei Zhu, Nian Wang, Peige Fang, Yuepeng Han, Ray Ming, Shaohua Li] Sugar content is a key feature of grape quality. The sugar content of grapes has been significantly improved after nearly a thousand years of artificial selection. However, the mechanism underlying the changes in the grape sugar content during the process of artificial selection remains largely unknown although several genes involved in sugar metabolism and transportation in grape have been identified. In this study, the genomes of 13 wild Vitis species and 14 cultivated Vitis vinifera accessions were resequenced to 2-5X depth using the Illumina Hiseq2000 platform. Genetic variation of 138 genes involved in sugar biosynthesis and transport was investigated, and 7,690 and 12,717 single nucleotide polymorphisms/insertions and deletions (SNPs/InDel) were identified within the cultivated V. vinifera and wild Vitis species, respectively. The percentages of SNPs/InDels were 0.93 and 1.54% in cultivated and wild species, respectively, and the wild Vitis species had 1.65-fold more SNPs/InDels than the cultivated V. vinifera. Moreover, the distribution of SNPs/InDels in gene regions was also investigated. Eight genes (HT4, PPFTK4, PPFTK6, PMT3, SPS1, HT8, HT15, SUSy1) showed low level of allelic diversity in cultivated species, suggesting they might have undergone purifying selection during the domestication process of grapes. Our genome DNA resequencing data provided a valuable resource for analyzing the effects of artificial selection on trait-related pathways in grape. The result that eight genes showed lower level of DNA variation in cultivated species than in wild species will be very helpful in understanding sugar accumulation in grapes. Springer-Verlag Berlin Heidelberg, 2013 Vitis vinifera nationallicence Wild Vitis species nationallicence Sugar nationallicence SNPs/InDels nationallicence Artificial selection nationallicence Xin Haiping Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences, 430074, Wuhan, People's Republic of China aut Zhang Jisen College of Life Sciences, Fujian Normal University, 350108, Fuzhou, People's Republic of China aut Zhu Wei Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences, 430074, Wuhan, People's Republic of China aut Wang Nian Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences, 430074, Wuhan, People's Republic of China aut Fang Peige Beijing Key Laboratory of Grape Sciences and Enology, Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences, Beijing, 100093, People's Republic of China aut Han Yuepeng Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences, 430074, Wuhan, People's Republic of China aut Ming Ray Department of Plant Biology, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA aut Li Shaohua Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences, 430074, Wuhan, People's Republic of China aut Tree Genetics & Genomes Springer Berlin Heidelberg 9/5(2013-10-01), 1343-1349 1614-2942 9:5<1343 2013 9 11295 https://doi.org/10.1007/s11295-013-0643-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0643-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xin Haiping Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences, 430074, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Jisen College of Life Sciences, Fujian Normal University, 350108, Fuzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Wei Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences, 430074, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Wang Nian Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences, 430074, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Fang Peige Beijing Key Laboratory of Grape Sciences and Enology, Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences, Beijing, 100093, People's Republic of China aut NATIONALLICENCE 700 1- Han Yuepeng Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences, 430074, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Ming Ray Department of Plant Biology, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Li Shaohua Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences, 430074, Wuhan, People's Republic of China aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer Berlin Heidelberg 9/5(2013-10-01), 1343-1349 1614-2942 9:5<1343 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer