naa a22 4500 510731031 CHVBK 20180411082952.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s11295-013-0617-9 doi (NATIONALLICENCE)springer-10.1007/s11295-013-0617-9 Development of male-specific SCAR marker in date palm ( Phoenix dactylifera L.) [Elektronische Daten] [Charu Dhawan, Pushpa Kharb, Richa Sharma, Sanjogta Uppal, Ramesh Aggarwal] Genetics of control mechanisms that underlies sex differentiation in date palm is not known. Sex of the plants becomes known only at the time of first flowering, which takes around 5years. In comparison, molecular diagnosis (if available/feasible) promises quick and reliable identification of sex types very early when plantlets are growing in seedbeds. To develop such an assay, genomic DNA from 45 individual plants (25 female and 20 male) belonging to different varieties of date palm was subjected to PCR amplification using 100 random amplified polymorphic DNA (RAPD) and 104 intersimple sequence repeat (ISSR) primers. Initially, two bulk genomic DNA samples (each made by pooling DNA from ten male and female plants, separately) were used. A primer showing sex-specific band in bulked samples was further used for amplification of the genomic DNA of the individual samples of that bulk. Only one RAPD primer, OPA-02, amplified a fragment of ~1.0kb in all the individual samples of male genotypes, whereas this fragment was absent in all the female genotypes. This male-specific fragment was cloned and sequenced (GenBank accession no. JN123357), and a sequence-characterized amplified region (SCAR) primer pair was designed that amplified a 406-bp fragment in both female and male genotypes and a unique fragment of 354bp in only male genotypes. The SCAR marker was further validated using 25 female and ten male date palm plants belonging to different varieties collected from different locations. Springer-Verlag Berlin Heidelberg, 2013 Date palm nationallicence Sex-specific marker nationallicence RAPD nationallicence ISSR nationallicence SCAR nationallicence Sex diagnosis nationallicence Dhawan Charu Department of Molecular Biology & Biotechnology, CCS Haryana Agricultural University, 125004, Hisar, India aut Kharb Pushpa Centre for Plant Biotechnology, CCS Haryana Agricultural University, 125004, Hisar, India aut Sharma Richa Molecular Marker Laboratory, Centre for Cellular and Molecular Biology (CSIR-CCMB), Uppal Road, 500007, Tarnaka, Hyderabad, India aut Uppal Sanjogta Department of Genetics, CCS Haryana Agricultural University, 125004, Hisar, India aut Aggarwal Ramesh Molecular Genetics Laboratory, Centre for Cellular and Molecular Biology (CSIR-CCMB), Uppal Road, 500007, Tarnaka, Hyderabad, India aut Tree Genetics & Genomes Springer Berlin Heidelberg 9/5(2013-10-01), 1143-1150 1614-2942 9:5<1143 2013 9 11295 https://doi.org/10.1007/s11295-013-0617-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0617-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dhawan Charu Department of Molecular Biology & Biotechnology, CCS Haryana Agricultural University, 125004, Hisar, India aut NATIONALLICENCE 700 1- Kharb Pushpa Centre for Plant Biotechnology, CCS Haryana Agricultural University, 125004, Hisar, India aut NATIONALLICENCE 700 1- Sharma Richa Molecular Marker Laboratory, Centre for Cellular and Molecular Biology (CSIR-CCMB), Uppal Road, 500007, Tarnaka, Hyderabad, India aut NATIONALLICENCE 700 1- Uppal Sanjogta Department of Genetics, CCS Haryana Agricultural University, 125004, Hisar, India aut NATIONALLICENCE 700 1- Aggarwal Ramesh Molecular Genetics Laboratory, Centre for Cellular and Molecular Biology (CSIR-CCMB), Uppal Road, 500007, Tarnaka, Hyderabad, India aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer Berlin Heidelberg 9/5(2013-10-01), 1143-1150 1614-2942 9:5<1143 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer