naa a22 4500 51073104X CHVBK 20180411082952.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s11295-013-0631-y doi (NATIONALLICENCE)springer-10.1007/s11295-013-0631-y Isolation, identification, and characterization of genomic LTR retrotransposon sequences from masson pine ( Pinus massoniana ) [Elektronische Daten] [Fuhua Fan, Xiaopeng Wen, Guijie Ding, Bowen Cui] Long terminal repeat (LTR) retrotransposons are ubiquitous in the plant kingdom and play an important role in plant genome evolution. Masson pine (Pinus massoniana) is one of the most economically important forest trees. However, the masson pine genome is poorly understood. Conserved domains of reverse transcriptase (RT) genes of Ty1-copia and Ty3-gypsy LTR retrotransposons were amplified from masson pine using degenerate primers. Sequence analysis showed less heterogeneity among Ty1-copia group retrotransposons than among Ty3-gypsy group retrotransposons. Phylogenetic analysis demonstrated that Ty1-copia and Ty3-gypsy RT sequences had considerable homology with other species, indicating that both vertical transmission and horizontal transmission may probably be the source of LTR retrotransposons in masson pine. Southern dot blot hybridization results suggested that both types of LTR retrotransposons are present in the genome of masson pine with high copy number. These results contribute to further understanding about the organization and evolution of the masson pine genome and provide fundamental genetic information for the utilization of retrotransposons in masson pine. Springer-Verlag Berlin Heidelberg, 2013 Retrotransposons nationallicence Reverse transcriptase nationallicence Heterogeneity nationallicence Masson pine nationallicence Blotting hybridization nationallicence Fan Fuhua Guizhou Key Laboratory of Agricultural Bioengineering, Guizhou University, 550025, Guiyang, Guizhou Province, People's Republic of China aut Wen Xiaopeng Guizhou Key Laboratory of Agricultural Bioengineering, Guizhou University, 550025, Guiyang, Guizhou Province, People's Republic of China aut Ding Guijie School of Forestry Science, Guizhou University, 550025, Guiyang, Guizhou Province, People's Republic of China aut Cui Bowen Guizhou Key Laboratory of Agricultural Bioengineering, Guizhou University, 550025, Guiyang, Guizhou Province, People's Republic of China aut Tree Genetics & Genomes Springer Berlin Heidelberg 9/5(2013-10-01), 1237-1246 1614-2942 9:5<1237 2013 9 11295 https://doi.org/10.1007/s11295-013-0631-y text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-013-0631-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fan Fuhua Guizhou Key Laboratory of Agricultural Bioengineering, Guizhou University, 550025, Guiyang, Guizhou Province, People's Republic of China aut NATIONALLICENCE 700 1- Wen Xiaopeng Guizhou Key Laboratory of Agricultural Bioengineering, Guizhou University, 550025, Guiyang, Guizhou Province, People's Republic of China aut NATIONALLICENCE 700 1- Ding Guijie School of Forestry Science, Guizhou University, 550025, Guiyang, Guizhou Province, People's Republic of China aut NATIONALLICENCE 700 1- Cui Bowen Guizhou Key Laboratory of Agricultural Bioengineering, Guizhou University, 550025, Guiyang, Guizhou Province, People's Republic of China aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer Berlin Heidelberg 9/5(2013-10-01), 1237-1246 1614-2942 9:5<1237 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer