naa a22 4500 510731090 CHVBK 20180411082953.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s11295-012-0561-0 doi (NATIONALLICENCE)springer-10.1007/s11295-012-0561-0 Pinus monticola pathogenesis-related gene PmPR10-2 alleles as defense candidates for stem quantitative disease resistance against white pine blister rust ( Cronartium ribicola ) [Elektronische Daten] [Jun-Jun Liu, Craig Hammett, Richard Sniezko] White pine blister rust, caused by the invasive fungus Cronartium ribicola, has been responsible for extremely high mortality of native western white pine (Pinus monticola) and other five-needle pines in natural stands throughout western North America. The presence of this non-native fungus has also led to greatly restricted use of western white pine for reforestation. A few families of defense proteins have been found as functional candidates involved in tree resistance to rust infection. Here we report genetic variation of a gene encoding a family 10 pathogenesis-related (PR) protein (PmPR10-2) in open-pollinated seed families with different levels of stem quantitative disease resistance (QDR). Six novel alleles and five common genotypes were identified inside the PmPR10-2 locus: these genetic variations included 33 single nucleotide polymorphisms (SNPs) throughout the gene regions and copy variation of a rare octanucleotide simple sequence repeat (SSR), 5′-AATTATTT-3′, in the gene intron. PmPR10-2 exhibited a moderate level (average r 2 = 0.42) of linkage disequilibrium. Two-thirds of the identified SNPs and the SSR marker were significantly associated with stem QDR levels. The PmPR10-2 genotype (H3:H3) exhibited the highest level of stem QDR (P < 0.01). Cost-effective and co-dominant SSR markers were developed and used for genotyping the PmPR10-2 locus using simple PCR, providing a potential molecular tool for accelerating screening efforts of white pine resistance against C. ribicola. Her Majesty the Queen in Right of Canada, 2012 Association analysis nationallicence Quantitative disease resistance (QDR) nationallicence Single nucleotide polymorphism (SNP) nationallicence Simple sequence repeat (SSR) nationallicence White pine blister rust (WPBR) nationallicence Liu Jun-Jun Pacific Forestry Centre, Canadian Forest Service, Natural Resources Canada, 506 West Burnside Road, V8Z 1M5, Victoria, BC, Canada aut Hammett Craig Pacific Forestry Centre, Canadian Forest Service, Natural Resources Canada, 506 West Burnside Road, V8Z 1M5, Victoria, BC, Canada aut Sniezko Richard Dorena Genetic Resource Center, USDA Forest Service, 34963 Shoreview Road, 97424, Cottage Grove, OR, USA aut Tree Genetics & Genomes Springer-Verlag 9/2(2013-04-01), 397-408 1614-2942 9:2<397 2013 9 11295 https://doi.org/10.1007/s11295-012-0561-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-012-0561-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Jun-Jun Pacific Forestry Centre, Canadian Forest Service, Natural Resources Canada, 506 West Burnside Road, V8Z 1M5, Victoria, BC, Canada aut NATIONALLICENCE 700 1- Hammett Craig Pacific Forestry Centre, Canadian Forest Service, Natural Resources Canada, 506 West Burnside Road, V8Z 1M5, Victoria, BC, Canada aut NATIONALLICENCE 700 1- Sniezko Richard Dorena Genetic Resource Center, USDA Forest Service, 34963 Shoreview Road, 97424, Cottage Grove, OR, USA aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer-Verlag 9/2(2013-04-01), 397-408 1614-2942 9:2<397 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer