naa a22 4500 510731139 CHVBK 20180411082953.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s11295-012-0581-9 doi (NATIONALLICENCE)springer-10.1007/s11295-012-0581-9 Genome size and ploidy levels in highly fragmented habitats: the case of western Mediterranean Juniperus (Cupressaceae) with special emphasis on J [Elektronische Daten] thurifera L [Angel Romo, Oriane Hidalgo, Adam Boratyński, Karolina Sobierajska, Anna Jasińska, Joan Vallès, Teresa Garnatje] Mediterranean junipers are of special ecological importance as key components of resource islands in semi-arid mountain ecosystems of the Mediterranean basin. The fragmentation of their habitat, which was primarily natural and driven by climatic drought conditions, is currently being aggravated by anthropogenic pressure. In the framework of this concern, the present work aims to contribute establishing a genomic profile of Juniperus in its western Mediterranean range, with a special emphasis placed on J. thurifera. DNA contents were assessed by flow cytometry in 43 populations of nine taxa within their Mediterranean range (first reports for J. navicularis, J. thurifera subsp. africana and J. thurifera subsp. thurifera). Chromosome numbers were determined by orcein staining in eight taxa (first counts for J. oxycedrus subsp. badia, J. phoenicea subsp. phoenicea, J. phoenicea subsp. turbinata, of 2n = 2x = 22, and for J. thurifera subsp. thurifera, of 2n = 4x = 44). Tetraploid cytotypes have been the only ones found in the 19 populations of J. thurifera studied, this being the first report of a Juniperus species exclusively polyploid. In J. thurifera, C-value does not respond to habitat fragmentation, in the same way that genetic diversity within populations was previously shown to be unaltered, suggesting that this factor has not had, at least to date, a significant impact on populations at genomic and genetic levels. Habitat fragmentation leads to deeply age-biased populations with a male-biased imbalanced sex ratio (lack of females), indicating an urgent need to improve regeneration within the populations of this species. Springer-Verlag Berlin Heidelberg, 2012 Flow cytometry nationallicence Gymnosperms nationallicence Habitat fragmentation nationallicence Juniper nationallicence Karyology nationallicence Polyploidy nationallicence Romo Angel Institut Botànic de Barcelona (IBB-CSIC-ICUB), Barcelona, Catalonia, Spain aut Hidalgo Oriane Laboratori de Botànica, Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Catalonia, Spain aut Boratyński Adam Polska Akademia Nauk, Instytut Dendrologii, Kórnik, Poland aut Sobierajska Karolina Polska Akademia Nauk, Instytut Dendrologii, Kórnik, Poland aut Jasińska Anna Polska Akademia Nauk, Instytut Dendrologii, Kórnik, Poland aut Vallès Joan Laboratori de Botànica, Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Catalonia, Spain aut Garnatje Teresa Institut Botànic de Barcelona (IBB-CSIC-ICUB), Barcelona, Catalonia, Spain aut Tree Genetics & Genomes Springer-Verlag 9/2(2013-04-01), 587-599 1614-2942 9:2<587 2013 9 11295 https://doi.org/10.1007/s11295-012-0581-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-012-0581-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Romo Angel Institut Botànic de Barcelona (IBB-CSIC-ICUB), Barcelona, Catalonia, Spain aut NATIONALLICENCE 700 1- Hidalgo Oriane Laboratori de Botànica, Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Catalonia, Spain aut NATIONALLICENCE 700 1- Boratyński Adam Polska Akademia Nauk, Instytut Dendrologii, Kórnik, Poland aut NATIONALLICENCE 700 1- Sobierajska Karolina Polska Akademia Nauk, Instytut Dendrologii, Kórnik, Poland aut NATIONALLICENCE 700 1- Jasińska Anna Polska Akademia Nauk, Instytut Dendrologii, Kórnik, Poland aut NATIONALLICENCE 700 1- Vallès Joan Laboratori de Botànica, Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Catalonia, Spain aut NATIONALLICENCE 700 1- Garnatje Teresa Institut Botànic de Barcelona (IBB-CSIC-ICUB), Barcelona, Catalonia, Spain aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer-Verlag 9/2(2013-04-01), 587-599 1614-2942 9:2<587 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer