naa a22 4500 510731147 CHVBK 20180411082953.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s11295-012-0559-7 doi (NATIONALLICENCE)springer-10.1007/s11295-012-0559-7 Transcriptome of oil palm ( Elaeis guineensis Jacq.) roots treated with Ganoderma boninense [Elektronische Daten] [Sue-Sean Tee, Yung-Chie Tan, Faridah Abdullah, Meilina Ong-Abdullah, Chai-Ling Ho] Basal stem rot (BSR) is the most devastating disease of oil palm. In this study, we examined the transcriptional responses of oil palm roots treated with a causal agent of BSR, Ganoderma boninense using a cDNA microarray approach. A total of 61 from 3,748 transcripts examined were found to be significantly up- or down-regulated in oil palm roots infected with G. boninense at 3 and 6weeks post inoculation compared to those from uninfected roots. The differentially expressed genes identified in the artificially infected oil palm roots included genes encoding isoflavone reductase, Em protein H2, SPX domain-containing protein 1, pathogenesis-related protein 1, vicilin-like antimicrobial peptide. The gene expression of isoflavone reductase, which is involved in the production of phytoalexin and three related genes in the phenylpropanoid biosynthetic pathway was also profiled in the treated oil palms using real-time quantitative reverse transcription PCR. This information has contributed to our understanding of the defense mechanisms of oil palm in response to G. boninense, the future development of molecular markers for marker assisted breeding and screening of oil palms that are tolerant to G. boninense. Springer-Verlag, 2012 cDNA microarray nationallicence Expression profiles nationallicence Ganoderma boninense nationallicence Oil palm nationallicence Phytoalexin nationallicence Tee Sue-Sean Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM-Serdang, Selangor, Malaysia aut Tan Yung-Chie Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400, UPM-Serdang, Selangor, Malaysia aut Abdullah Faridah Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400, UPM-Serdang, Selangor, Malaysia aut Ong-Abdullah Meilina Advanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board (MPOB), 6 Persiaran Institusi, Bandar Baru Bangi, Selangor, Malaysia aut Ho Chai-Ling Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM-Serdang, Selangor, Malaysia aut Tree Genetics & Genomes Springer-Verlag 9/2(2013-04-01), 377-386 1614-2942 9:2<377 2013 9 11295 https://doi.org/10.1007/s11295-012-0559-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-012-0559-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tee Sue-Sean Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM-Serdang, Selangor, Malaysia aut NATIONALLICENCE 700 1- Tan Yung-Chie Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400, UPM-Serdang, Selangor, Malaysia aut NATIONALLICENCE 700 1- Abdullah Faridah Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400, UPM-Serdang, Selangor, Malaysia aut NATIONALLICENCE 700 1- Ong-Abdullah Meilina Advanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board (MPOB), 6 Persiaran Institusi, Bandar Baru Bangi, Selangor, Malaysia aut NATIONALLICENCE 700 1- Ho Chai-Ling Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM-Serdang, Selangor, Malaysia aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer-Verlag 9/2(2013-04-01), 377-386 1614-2942 9:2<377 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer