naa a22 4500 510731236 CHVBK 20180411082953.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s11295-012-0576-6 doi (NATIONALLICENCE)springer-10.1007/s11295-012-0576-6 A physical map of the Chinese chestnut ( Castanea mollissima ) genome and its integration with the genetic map [Elektronische Daten] [Guang-Chen Fang, Barbara Blackmon, Margaret Staton, C. Nelson, Thomas Kubisiak, Bode Olukolu, David Henry, Tatyana Zhebentyayeva, Christopher Saski, Chun-Huai Cheng, Megan Monsanto, Stephen Ficklin, Michael Atkins, Laura Georgi, Abdelali Barakat, Nicholas Wheeler, John Carlson, Ronald Sederoff, Albert Abbott] Three Chinese chestnut bacterial artificial chromosome (BAC) libraries were developed and used for physical map construction. Specifically, high information content fingerprinting was used to assemble 126,445 BAC clones into 1,377 contigs and 12,919 singletons. Integration of the dense Chinese chestnut genetic map with the physical map was achieved via high-throughput hybridization using overgo probes derived from sequence-based genetic markers. A total of 1,026 probes were anchored to the physical map including 831 probes corresponding to 878 expressed sequence tag-based markers. Within the physical map, three BAC contigs were anchored to the three major fungal blight-resistant quantitative trait loci on chestnut linkage groups B, F, and G. A subset of probes corresponding to orthologous genes in poplar showed only a limited amount of conserved gene order between the poplar and chestnut genomes. The integrated genetic and physical map of Chinese chestnut is available at www.fagaceae.org/physical_maps . The Author(s), 2012 Castanea spp nationallicence Chestnut nationallicence BAC library nationallicence HICF fingerprinting nationallicence Physical map nationallicence Comparative genomics nationallicence Chestnut blight resistance nationallicence Fang Guang-Chen USDA APHIS BRS, 920 Main Campus Drive, Suite 200, 27606, Raleigh, NC, USA aut Blackmon Barbara Clemson University Genomics Institute, Clemson University, BRC, 105 Collings Street, 29634, Clemson, SC, USA aut Staton Margaret Clemson University Genomics Institute, Clemson University, BRC, 105 Collings Street, 29634, Clemson, SC, USA aut Nelson C. Southern Institute of Forest Genetics, U.S. Forest Service, 23332 Success Road, 39574, Saucier, MS, USA aut Kubisiak Thomas Southern Institute of Forest Genetics, U.S. Forest Service, 23332 Success Road, 39574, Saucier, MS, USA aut Olukolu Bode Department of Plant Pathology, North Carolina State University, 27695, Raleigh, NC, USA aut Henry David Piedmont Technical College, 620 North Emerald Road, 29648, Greenwood, SC, USA aut Zhebentyayeva Tatyana Department of Genetics and Biochemistry, Clemson University, 100 Jordan Hall, 29634, Clemson, SC, USA aut Saski Christopher Clemson University Genomics Institute, Clemson University, BRC, 105 Collings Street, 29634, Clemson, SC, USA aut Cheng Chun-Huai Department of Horticulture, Washington State University, Johnson Hall 149, 99164, Pullman, WA, USA aut Monsanto Megan SDSU Heart Institute and Department of Biology, San Diego State University, 5500 Campanile Drive, 92182, San Diego, CA, USA aut Ficklin Stephen Department of Horticulture, Washington State University, Johnson Hall 149, 99164, Pullman, WA, USA aut Atkins Michael Clemson University Genomics Institute, Clemson University, BRC, 105 Collings Street, 29634, Clemson, SC, USA aut Georgi Laura Meadowview Research Farms, The American Chestnut Foundation, 29010 Hawthorne Drive, 24361, Meadowview, VA, USA aut Barakat Abdelali The School of Forest Resources, The Huck Institutes of the Life Sciences, Pennsylvania State University, 324 Forest Resources Building, 16802, University Park, PA, USA aut Wheeler Nicholas Department of Forestry and Environmental Resources, North Carolina State University, Campus Box 8001, 27695, Raleigh, NC, USA aut Carlson John The School of Forest Resources, The Huck Institutes of the Life Sciences, Pennsylvania State University, 324 Forest Resources Building, 16802, University Park, PA, USA aut Sederoff Ronald Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Campus Box 7247, 27695, Raleigh, NC, USA aut Abbott Albert Department of Genetics and Biochemistry, Clemson University, 100 Jordan Hall, 29634, Clemson, SC, USA aut Tree Genetics & Genomes Springer-Verlag 9/2(2013-04-01), 525-537 1614-2942 9:2<525 2013 9 11295 https://doi.org/10.1007/s11295-012-0576-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-012-0576-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fang Guang-Chen USDA APHIS BRS, 920 Main Campus Drive, Suite 200, 27606, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Blackmon Barbara Clemson University Genomics Institute, Clemson University, BRC, 105 Collings Street, 29634, Clemson, SC, USA aut NATIONALLICENCE 700 1- Staton Margaret Clemson University Genomics Institute, Clemson University, BRC, 105 Collings Street, 29634, Clemson, SC, USA aut NATIONALLICENCE 700 1- Nelson C. Southern Institute of Forest Genetics, U.S. Forest Service, 23332 Success Road, 39574, Saucier, MS, USA aut NATIONALLICENCE 700 1- Kubisiak Thomas Southern Institute of Forest Genetics, U.S. Forest Service, 23332 Success Road, 39574, Saucier, MS, USA aut NATIONALLICENCE 700 1- Olukolu Bode Department of Plant Pathology, North Carolina State University, 27695, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Henry David Piedmont Technical College, 620 North Emerald Road, 29648, Greenwood, SC, USA aut NATIONALLICENCE 700 1- Zhebentyayeva Tatyana Department of Genetics and Biochemistry, Clemson University, 100 Jordan Hall, 29634, Clemson, SC, USA aut NATIONALLICENCE 700 1- Saski Christopher Clemson University Genomics Institute, Clemson University, BRC, 105 Collings Street, 29634, Clemson, SC, USA aut NATIONALLICENCE 700 1- Cheng Chun-Huai Department of Horticulture, Washington State University, Johnson Hall 149, 99164, Pullman, WA, USA aut NATIONALLICENCE 700 1- Monsanto Megan SDSU Heart Institute and Department of Biology, San Diego State University, 5500 Campanile Drive, 92182, San Diego, CA, USA aut NATIONALLICENCE 700 1- Ficklin Stephen Department of Horticulture, Washington State University, Johnson Hall 149, 99164, Pullman, WA, USA aut NATIONALLICENCE 700 1- Atkins Michael Clemson University Genomics Institute, Clemson University, BRC, 105 Collings Street, 29634, Clemson, SC, USA aut NATIONALLICENCE 700 1- Georgi Laura Meadowview Research Farms, The American Chestnut Foundation, 29010 Hawthorne Drive, 24361, Meadowview, VA, USA aut NATIONALLICENCE 700 1- Barakat Abdelali The School of Forest Resources, The Huck Institutes of the Life Sciences, Pennsylvania State University, 324 Forest Resources Building, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Wheeler Nicholas Department of Forestry and Environmental Resources, North Carolina State University, Campus Box 8001, 27695, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Carlson John The School of Forest Resources, The Huck Institutes of the Life Sciences, Pennsylvania State University, 324 Forest Resources Building, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Sederoff Ronald Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Campus Box 7247, 27695, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Abbott Albert Department of Genetics and Biochemistry, Clemson University, 100 Jordan Hall, 29634, Clemson, SC, USA aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer-Verlag 9/2(2013-04-01), 525-537 1614-2942 9:2<525 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer