naa a22 4500 510731252 CHVBK 20180411082953.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s11295-012-0569-5 doi (NATIONALLICENCE)springer-10.1007/s11295-012-0569-5 A second-generation diagnostic single nucleotide polymorphism (SNP)-based assay, optimized to distinguish among eight poplar ( Populus L.) species and their early hybrids [Elektronische Daten] [Nathalie Isabel, Manuel Lamothe, Stacey Thompson] Rapid identification of Populus L. species and hybrids can be achieved with relatively little effort through the use of primer extension-based single nucleotide polymorphism (SNP) genotyping assays. We present an optimized set of 36 SNP markers from 28 gene regions that diagnose eight poplar species (Populus angustifolia James, Populus balsamifera L., Populus deltoides Bartram, Populus fremontii Watson, Populus laurifolia Ledeb., Populus maximowiczii Henry, Populus nigra L., and Populus trichocarpa Torr. & Gray). A total of 700 DNA sequences from six Populus species (1-15 individuals per species) were used to construct the array. A set of flanking and probe oligonucleotides was developed and tested. The accuracy of the SNP assay was validated by genotyping 448 putatively "pure” individuals from 14 species of Populus. Overall, the SNP assay had a high success rate (97.6%) and will prove useful for the identification of all Aigeiros Duby and Tacamahaca Spach. species and their early-generation hybrids within natural populations and breeding programs. Null alleles and intraspecific polymorphisms were detected for a few locus/species combinations in the Aigeiros and Tacamahaca sections. When we attempted to genotype aspens of the section Populus (Populus alba L., Populus grandidentata Michx., Populus tremula L., and Populus tremuloides Michx.), the success rate of the SNP array decreased by 13%, demonstrating moderate cross-sectional transferability. The Author(s), 2012 Genotyping array nationallicence Hybridization nationallicence Marker development nationallicence Poplars nationallicence Clone certification nationallicence Species identification nationallicence Isabel Nathalie Laurentian Forestry Centre, Canadian Forest Service, Natural Resources Canada, 1055, rue du P.E.P.S., C. P. 10380, Succ. Sainte-Foy, G1V 4C7, Québec, QC, Canada aut Lamothe Manuel Laurentian Forestry Centre, Canadian Forest Service, Natural Resources Canada, 1055, rue du P.E.P.S., C. P. 10380, Succ. Sainte-Foy, G1V 4C7, Québec, QC, Canada aut Thompson Stacey Laurentian Forestry Centre, Canadian Forest Service, Natural Resources Canada, 1055, rue du P.E.P.S., C. P. 10380, Succ. Sainte-Foy, G1V 4C7, Québec, QC, Canada aut Tree Genetics & Genomes Springer-Verlag 9/2(2013-04-01), 621-626 1614-2942 9:2<621 2013 9 11295 https://doi.org/10.1007/s11295-012-0569-5 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-012-0569-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Isabel Nathalie Laurentian Forestry Centre, Canadian Forest Service, Natural Resources Canada, 1055, rue du P.E.P.S., C. P. 10380, Succ. Sainte-Foy, G1V 4C7, Québec, QC, Canada aut NATIONALLICENCE 700 1- Lamothe Manuel Laurentian Forestry Centre, Canadian Forest Service, Natural Resources Canada, 1055, rue du P.E.P.S., C. P. 10380, Succ. Sainte-Foy, G1V 4C7, Québec, QC, Canada aut NATIONALLICENCE 700 1- Thompson Stacey Laurentian Forestry Centre, Canadian Forest Service, Natural Resources Canada, 1055, rue du P.E.P.S., C. P. 10380, Succ. Sainte-Foy, G1V 4C7, Québec, QC, Canada aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer-Verlag 9/2(2013-04-01), 621-626 1614-2942 9:2<621 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer