naa a22 4500 510731325 CHVBK 20180411082954.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s11295-012-0579-3 doi (NATIONALLICENCE)springer-10.1007/s11295-012-0579-3 A transcriptome-based genetic map of Chinese chestnut ( Castanea mollissima ) and identification of regions of segmental homology with peach ( Prunus persica ) [Elektronische Daten] [T. Kubisiak, C. Nelson, M. Staton, T. Zhebentyayeva, C. Smith, B. Olukolu, G.-C. Fang, F. Hebard, S. Anagnostakis, N. Wheeler, P. Sisco, A. Abbott, R. Sederoff] The Chinese chestnut (Castanea mollissima) carries resistance to Cryphonectria parasitica, the fungal pathogen inciting chestnut blight. The pathogen, introduced from Asia, devastated the American chestnut (Castanea dentata) throughout its native range early in the twentieth century. A highly informative genetic map of Chinese chestnut was constructed to extend genomic studies in the Fagaceae and to aid the introgression of Chinese chestnut blight resistance genes into American chestnut. Two mapping populations were established with three Chinese chestnut parents, ‘Mahogany', ‘Nanking', and ‘Vanuxem', totaling 337 progeny. The transcriptome-based genetic map was created with 329 simple sequence repeat and 1,064 single nucleotide polymorphism markers all derived from expressed sequence tag sequences. Genetic maps for each parent were developed and combined to establish 12 consensus linkage groups spanning 742cM, providing the the most comprehensive genetic map for a Fagaceae species to date. Over 75% of the mapped markers from the Chinese chestnut consensus genetic map were placed on the physical map using overgo hybridization, providing a fully integrated genetic and physical map resource for Castanea spp. About half (57%) of the Chinese chestnut genetic map could be assigned to regions of segmental homology with 58% of the peach (Prunus persica) genome assembly. A three quantitative trait loci (QTL) model for blight resistance was verified using the new genetic markers and an existing interspecies (C. mollissima × C. dentata) F2 mapping population. Two of the blight resistance QTLs in chestnut shared synteny with two QTLs for powdery mildew resistance in peach, indicating the potential conservation of disease resistance genes at these loci. The Author(s), 2012 Fagaceae nationallicence Castanea spp nationallicence Chestnut nationallicence Genetic map nationallicence Comparative genomics nationallicence Disease resistance nationallicence Kubisiak T. Southern Institute of Forest Genetics, Southern Research Station, U.S. Forest Service, 39574, Saucier, MS, USA aut Nelson C. Southern Institute of Forest Genetics, Southern Research Station, U.S. Forest Service, 39574, Saucier, MS, USA aut Staton M. Clemson University Genomics Institute, Clemson University, 29634, Clemson, SC, USA aut Zhebentyayeva T. Department of Genetics and Biochemistry, Clemson University, 29634, Clemson, SC, USA aut Smith C. Bioinformatics Research Center, North Carolina State University, 27695, Raleigh, NC, USA aut Olukolu B. Department of Genetics and Biochemistry, Clemson University, 29634, Clemson, SC, USA aut Fang G.-C Clemson University Genomics Institute, Clemson University, 29634, Clemson, SC, USA aut Hebard F. Meadowview Research Farm, The American Chestnut Foundation, 24361, Meadowview, VA, USA aut Anagnostakis S. Connecticut Agricultural Experiment Station, 06504, New Haven, CT, USA aut Wheeler N. Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, 27695, Raleigh, NC, USA aut Sisco P. The American Chestnut Foundation, 28801, Asheville, NC, USA aut Abbott A. Department of Genetics and Biochemistry, Clemson University, 29634, Clemson, SC, USA aut Sederoff R. Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, 27695, Raleigh, NC, USA aut Tree Genetics & Genomes Springer-Verlag 9/2(2013-04-01), 557-571 1614-2942 9:2<557 2013 9 11295 https://doi.org/10.1007/s11295-012-0579-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-012-0579-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kubisiak T. Southern Institute of Forest Genetics, Southern Research Station, U.S. Forest Service, 39574, Saucier, MS, USA aut NATIONALLICENCE 700 1- Nelson C. Southern Institute of Forest Genetics, Southern Research Station, U.S. Forest Service, 39574, Saucier, MS, USA aut NATIONALLICENCE 700 1- Staton M. Clemson University Genomics Institute, Clemson University, 29634, Clemson, SC, USA aut NATIONALLICENCE 700 1- Zhebentyayeva T. Department of Genetics and Biochemistry, Clemson University, 29634, Clemson, SC, USA aut NATIONALLICENCE 700 1- Smith C. Bioinformatics Research Center, North Carolina State University, 27695, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Olukolu B. Department of Genetics and Biochemistry, Clemson University, 29634, Clemson, SC, USA aut NATIONALLICENCE 700 1- Fang G.-C Clemson University Genomics Institute, Clemson University, 29634, Clemson, SC, USA aut NATIONALLICENCE 700 1- Hebard F. Meadowview Research Farm, The American Chestnut Foundation, 24361, Meadowview, VA, USA aut NATIONALLICENCE 700 1- Anagnostakis S. Connecticut Agricultural Experiment Station, 06504, New Haven, CT, USA aut NATIONALLICENCE 700 1- Wheeler N. Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, 27695, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Sisco P. The American Chestnut Foundation, 28801, Asheville, NC, USA aut NATIONALLICENCE 700 1- Abbott A. Department of Genetics and Biochemistry, Clemson University, 29634, Clemson, SC, USA aut NATIONALLICENCE 700 1- Sederoff R. Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, 27695, Raleigh, NC, USA aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer-Verlag 9/2(2013-04-01), 557-571 1614-2942 9:2<557 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer