naa a22 4500 510733778 CHVBK 20180411083002.0 cr unu---uuuuu 180411e20131101xx s 000 0 eng 10.1007/s12039-013-0525-4 doi (NATIONALLICENCE)springer-10.1007/s12039-013-0525-4 Purification and characterization of laccase from Coriolopsis floccosa MTCC-1177 and its use in the selective oxidation of aromatic methyl group to aldehyde without mediators [Elektronische Daten] [P CHAURASIA, A YADAV, R YADAV, S YADAVA] A laccase from the culture filtrate of white rot fungus Coriolopsis floccosa MTCC-1177 has been purified to homogeneity. The method involved concentration of the culture filtrate by ammonium sulphate precipitation and an anion exchange chromatography on diethylaminoethyl (DEAE) cellulose. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) as well as native polyacrylamide gel electrophoresis (native-PAGE) produced single protein bands indicating that the enzyme preparation was pure. Molecular mass of the enzyme determined from SDS-PAGE analysis was 64 kDa. Using 2,6-dimethoxyphenol (DMP), 2,2′-[Azino-bis-(3-ethylbonzthiazoline-6-sulphonic acid (ABTS) diammonium salt and 4-hydroxy-3,5-dimethoxy benzaldehyde azine (syringaldazine) as the substrates, the K m, k cat and k cat/K m values of the laccase were found to be 112.5 μM, 5.16 s−1, 4.60 × 104 M − 1 s−1, 58 μM, 5.16 s−1, 8.90 × 104 M − 1 s−1 and 100 μM, 5.16 s−1, 5.16× 104 M − 1 s−1, respectively. The pH and temperature optima were 5.0°C and 40°C, respectively. Activation energy for thermal denaturation of the enzyme was 36.6 kJ/mol/K. The enzyme was most stable at pH 4.0 when exposed for 1 h. The purified laccase has yellow colour and does not show absorption band around 610 nm found in blue laccases. The enzyme transforms toluene, 3-nitrotoluene and 4-chlorotoluene to benzaldehyde, 3-nitrobenzaldehyde and 4-chlorobenzaldehyde, respectively, in the absence of electron transfer mediators. Graphical Abstract A yellow laccase has been purified and characterized from the culture filtrate of white rot fungus Coriolopsis floccosa MTCC-1177. This laccase selectively transforms the aromatic methyl group to aromatic aldehyde group in the absence of mediator molecules. The average yield of the products was 90%. Indian Academy of Sciences, 2013 Cu enzymes nationallicence yellow laccases nationallicence lignolytic enzymes nationallicence Coriolopsis floccosa nationallicence 2,6- dimethoxyphenol nationallicence white rot fungus nationallicence CHAURASIA P. Department of Chemistry, DDU Gorakhpur University, 273 009, Gorakhpur, India aut YADAV A. Department of Chemistry, DDU Gorakhpur University, 273 009, Gorakhpur, India aut YADAV R. Department of Chemistry, DDU Gorakhpur University, 273 009, Gorakhpur, India aut YADAVA S. Department of Chemistry, DDU Gorakhpur University, 273 009, Gorakhpur, India aut Journal of Chemical Sciences Springer India 125/6(2013-11-01), 1395-1403 0974-3626 125:6<1395 2013 125 12039 https://doi.org/10.1007/s12039-013-0525-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12039-013-0525-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- CHAURASIA P. Department of Chemistry, DDU Gorakhpur University, 273 009, Gorakhpur, India aut NATIONALLICENCE 700 1- YADAV A. Department of Chemistry, DDU Gorakhpur University, 273 009, Gorakhpur, India aut NATIONALLICENCE 700 1- YADAV R. Department of Chemistry, DDU Gorakhpur University, 273 009, Gorakhpur, India aut NATIONALLICENCE 700 1- YADAVA S. Department of Chemistry, DDU Gorakhpur University, 273 009, Gorakhpur, India aut NATIONALLICENCE 773 0- Journal of Chemical Sciences Springer India 125/6(2013-11-01), 1395-1403 0974-3626 125:6<1395 2013 125 12039 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer