naa a22 4500 510734243 CHVBK 20180411083004.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12039-013-0434-6 doi (NATIONALLICENCE)springer-10.1007/s12039-013-0434-6 Activity, stability and kinetic parameters for α -chymotrypsin catalysed reactions in AOT/isooctane reverse micelles with nonionic and zwitterionic mixed surfactants [Elektronische Daten] [SANTOSH VERMA, KALLOL GHOSH] Reverse micelles (RMs) of sodium 1,4-bis(2-ethylhexyl)sulphosuccinate (AOT) in nonpolar organic solvents are widely known to have very high solubilization power for water. The method is applied to the hydrolysis of p-nitrophenyl acetate (PNPA) catalysed by α-chymotrypsin (α-CT) in AOT/isooctane/buffer RMs. The increase in α-CT activity and stability was an optimum at w o ([H2O]/[AOT]) = 10, z [Isooctane]/[AOT]) = 5. Three typical surfactants were selected based on their head group charges: a non-ionic surfactant Triton-X 100 and two zwitterionic sulphobetaine surfactants of the type C n H2n + 1N + Me2 (CH $_{2})_{3\thinspace }$ SO $_{3}^{-}$ (n = 10; SB3-10, n = 16; SB3-16). The kinetic parameters (such as k cat and K M) of the α-CT at 27°C were determined and compared in the absence and presence of three surfactants. The effect of chain length of zwitterionic surfactant (SB3-10 and SB3-16) on the enzymatic efficacy of α-CT as a function of mixed surfactant addition has been investigated in AOT/isooctane RMs at pH 7.75. Graphical Abstract The α-chymotrypsin (α-CT) hydrolysis of p-nitrophenyl acetate (PNPA) in AOT/isooctane/buffer reverse micelles (RMs) have been studied with varying water content, wo, in the presence of Triton-X 100 and sulphobetaine surfactants. The activity of α-CT depends on the water pool size and the amount of AOT and mixed surfactant media. Indian Academy of Sciences, 2013 α  −Chymotrypsin nationallicence stability nationallicence p -nitrophenyl acetate nationallicence zwitterionic surfactants nationallicence reverse micelles nationallicence VERMA SANTOSH Shri Shankaracharya Institute of Technology and Management, 490 020, Bhilai, India aut GHOSH KALLOL School of Studies in Chemistry, Pt. Ravishankar Shukla University, 492 010, Raipur, India aut Journal of Chemical Sciences Springer India 125/4(2013-07-01), 875-882 0974-3626 125:4<875 2013 125 12039 https://doi.org/10.1007/s12039-013-0434-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12039-013-0434-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- VERMA SANTOSH Shri Shankaracharya Institute of Technology and Management, 490 020, Bhilai, India aut NATIONALLICENCE 700 1- GHOSH KALLOL School of Studies in Chemistry, Pt. Ravishankar Shukla University, 492 010, Raipur, India aut NATIONALLICENCE 773 0- Journal of Chemical Sciences Springer India 125/4(2013-07-01), 875-882 0974-3626 125:4<875 2013 125 12039 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer