naa a22 4500 510734359 CHVBK 20180411083004.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12039-013-0440-8 doi (NATIONALLICENCE)springer-10.1007/s12039-013-0440-8 Antileishmanial polyphenols from Corymbia maculata [Elektronische Daten] [JASMEEN SIDANA, DINESH NEERADI, ALKA CHOUDHARY, SUSHMA SINGH, WILLIAM FOLEY, INDER SINGH] An activity-guided fractionation was used to identify the antileishmanial compounds of Corymbia maculata. The hexane, ethyl acetate and methanol extracts were active in in vitro antileishmanial assay. Twelve polyphenols including 8-demethyl eucalyptin (1), eucalyptin (2), myrciaphenone A (3), myrciaphenone B (4), quercetin-3-O-β-d-xylopyranoside (5), myricetin-3-O-α-l-rhamnopyranoside (6), quercetin-3-O-β-d-galactopyranoside (7), quercetin-3-O-β-d-glucopyranoside (8), quercetin-3-O-α-l-rhamnopyranoside (9), syringic acid (10), gallic acid-3-methyl ether (11), gallic acid-4-methyl ether (12) and gallic acid (13) were isolated from the active extracts. All the tested compounds except 8-demethyleucalyptin and myrciaphenone B showed strong to moderate (6.9-24.5 $\upmu$ M) antileishmanial activity against Leishmania donovani promastigotes. An HPLC-PDA method has been developed to detect/quantify 29 compounds in the extracts of C. maculata leaves. This validated method allows simultaneous quantitation of seven flavonoids, fourteen phloroglucinols and eight other polyphenols and can be applied for qualitative as well as quantitative determination of phytoconstituents in Eucalyptus matrices. Graphical Abstract Phytochemical screening of Corymbia maculata resulted in the isolation of thirteen compounds from hexane and methanol extracts of the dried leaves. Flavonoid glycosides and a phloroglucinol glycoside were the active antileishmanial compounds. An HPLC-PDA method was developed and applied to detect and quantify twelve compounds in the extracts of C. maculata leaves. Indian Academy of Sciences, 2013 Corymbia maculata nationallicence flavonoid glycosides nationallicence methylated flavonoids nationallicence myrciaphenone nationallicence HPLC quantitation nationallicence SIDANA JASMEEN Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, SAS Nagar, 160 062, Punjab, India aut NEERADI DINESH Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, SAS Nagar, 160 062, Punjab, India aut CHOUDHARY ALKA Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, SAS Nagar, 160 062, Punjab, India aut SINGH SUSHMA Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, SAS Nagar, 160 062, Punjab, India aut FOLEY WILLIAM Research School of Biology, The Australian National University, 0200, Canberra, Australia aut SINGH INDER Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, SAS Nagar, 160 062, Punjab, India aut Journal of Chemical Sciences Springer India 125/4(2013-07-01), 765-775 0974-3626 125:4<765 2013 125 12039 https://doi.org/10.1007/s12039-013-0440-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12039-013-0440-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- SIDANA JASMEEN Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, SAS Nagar, 160 062, Punjab, India aut NATIONALLICENCE 700 1- NEERADI DINESH Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, SAS Nagar, 160 062, Punjab, India aut NATIONALLICENCE 700 1- CHOUDHARY ALKA Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, SAS Nagar, 160 062, Punjab, India aut NATIONALLICENCE 700 1- SINGH SUSHMA Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, SAS Nagar, 160 062, Punjab, India aut NATIONALLICENCE 700 1- FOLEY WILLIAM Research School of Biology, The Australian National University, 0200, Canberra, Australia aut NATIONALLICENCE 700 1- SINGH INDER Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, SAS Nagar, 160 062, Punjab, India aut NATIONALLICENCE 773 0- Journal of Chemical Sciences Springer India 125/4(2013-07-01), 765-775 0974-3626 125:4<765 2013 125 12039 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer