naa a22 4500 510734766 CHVBK 20180411083005.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s12039-013-0452-4 doi (NATIONALLICENCE)springer-10.1007/s12039-013-0452-4 ARULSAMY ANDREW Condensed Matter Group, Division of Interdisciplinary Science, F-02-08 Ketumbar Hill, 56100, Jalan Ketumbar, Kuala-Lumpur, Malaysia aut Nucleotide insertion initiated by van der Waals interaction during polymerase beta DNA replication [Elektronische Daten] [ANDREW ARULSAMY] We present here an unambiguous theoretical analyses and to show that the exclusive biochemical reaction involved in a single nucleotide insertion into the DNA primer can be efficiently tracked using the renormalized van der Waals (vdW) interaction of a stronger type, the Hermansson blue-shifting hydrogen bond effect, and the Arunan composite hydrogen-vdW bond. We find that there are two biochemical steps involved to complete the insertion of a single base (cytosine) into the 3′ end of a DNA primer. First, the O3′ (from a DNA primer) initiates the nucleophilic attack on P α (from an incoming dCTP), in response, O3 α (bonded to P α ) interacts with H′ (bonded to O3′). These interactions are shown to be strongly interdependent and require the forming and breaking of P—O and H—O covalent bonds, which in turn imply that we do not need any external energy supply. Graphical Abstract There are two processes responsible for a single base (C) insertion into the 3′ end of the DNA primer, depicted by steps A1 and A3α. We have proven that these two steps have the strongest Coulomb and van der Waals lock-in interaction, which are sufficient to initiate the nucleotide insertion. Indian Academy of Sciences, 2013 Interacting biomolecules nationallicence peptide-bond formation nationallicence generalized van der Waals interaction nationallicence ionization energy theory nationallicence Journal of Chemical Sciences Springer India 125/5(2013-09-01), 1223-1235 0974-3626 125:5<1223 2013 125 12039 https://doi.org/10.1007/s12039-013-0452-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12039-013-0452-4 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- ARULSAMY ANDREW Condensed Matter Group, Division of Interdisciplinary Science, F-02-08 Ketumbar Hill, 56100, Jalan Ketumbar, Kuala-Lumpur, Malaysia aut NATIONALLICENCE 773 0- Journal of Chemical Sciences Springer India 125/5(2013-09-01), 1223-1235 0974-3626 125:5<1223 2013 125 12039 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer