naa a22 4500 510734936 CHVBK 20180411083006.0 cr unu---uuuuu 180411e20130301xx s 000 0 eng 10.1007/s12039-013-0379-9 doi (NATIONALLICENCE)springer-10.1007/s12039-013-0379-9 Electrochemistry of surface wired cytochrome c and bioelectrocatalytic sensing of superoxide [Elektronische Daten] [SUSMITA BEHERA, RAMENDRA DEY, MANAS RANA, C RAJ] Electrochemistry of cytochrome c (Cyt-c) wired on an electrode modified with the self-assemblies of 4,4′-dithio-dibutyric acid (DTB) and 2-pyrazineethane thiol (PET) by covalent and electrostatic binding and the amperometric sensing of superoxide (O $_{2}^{-})$ are described. Cyt-c wired on the mixed self-assembly of DTB and PET displays well-defined voltammetric response at 0.025 V with a peak-to-peak separation (ΔE p ) of 5 mV. Pyrazine unit in the mixed self-assembly promotes the electron transfer in the redox reaction of surface wired Cyt-c. Cyt-c wired on the mixed self-assembly has been used for the amperometric sensing of superoxide. The enzymatically generated superoxide has been successfully detected using the Cyt-c wired electrode. High sensitivity and fast response for superoxide have been achieved. Uric acid does not interfere in the amperometric measurement of superoxide. The interference due to H2O2 has been eliminated by using enzyme catalase. Graphical Abstract Wiring of cytochrome c on the mixed self-assembly of 4,4'-dithio-dibutyric acid and 2-pyrazineethane thiol and the amperometric sensing of superoxide are described. Indian Academy of Sciences, 2013 Mixed self-assembled monolayer nationallicence pyrazine nationallicence cytochrome c nationallicence superoxide sensing nationallicence amperometry nationallicence BEHERA SUSMITA Department of Chemistry, Indian Institute of Technology, 721 302, Kharagpur, India aut DEY RAMENDRA Department of Chemistry, Indian Institute of Technology, 721 302, Kharagpur, India aut RANA MANAS Department of Chemistry, Indian Institute of Technology, 721 302, Kharagpur, India aut RAJ C. Department of Chemistry, Indian Institute of Technology, 721 302, Kharagpur, India aut Journal of Chemical Sciences Springer-Verlag 125/2(2013-03-01), 275-282 0974-3626 125:2<275 2013 125 12039 https://doi.org/10.1007/s12039-013-0379-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12039-013-0379-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- BEHERA SUSMITA Department of Chemistry, Indian Institute of Technology, 721 302, Kharagpur, India aut NATIONALLICENCE 700 1- DEY RAMENDRA Department of Chemistry, Indian Institute of Technology, 721 302, Kharagpur, India aut NATIONALLICENCE 700 1- RANA MANAS Department of Chemistry, Indian Institute of Technology, 721 302, Kharagpur, India aut NATIONALLICENCE 700 1- RAJ C. Department of Chemistry, Indian Institute of Technology, 721 302, Kharagpur, India aut NATIONALLICENCE 773 0- Journal of Chemical Sciences Springer-Verlag 125/2(2013-03-01), 275-282 0974-3626 125:2<275 2013 125 12039 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer