naa a22 4500 510735037 CHVBK 20180411083006.0 cr unu---uuuuu 180411e20130301xx s 000 0 eng 10.1007/s12039-013-0388-8 doi (NATIONALLICENCE)springer-10.1007/s12039-013-0388-8 Role of N -methyl-8-(alkoxy)quinolinium iodide in suppression of protein-protein interactions [Elektronische Daten] [BIMLESH OJHA, CHIRANTAN KAR, GOPAL DAS] There is a great deal of interest in developing small molecule inhibitors of protein misfolding and aggregation due to a growing number of pathologic states known as amyloid disorders. In searching for alternative ways to reduce protein-protein interactions or to inhibit the amyloid formation, the inhibitory effects of cationic amphiphile viz. N-methyl-8-(alkoxy)quinolinium iodide on aggregation behaviour of hen egg white lysozyme (HEWL) at alkaline pH has been studied. Even though the compounds did not protect native HEWL from conformational changes, they were effective in diminishing HEWL amyloid formation, delaying both nucleation and elongation phases. It is likely that strong binding in the HEWL compound complex, raises the activation energy barrier for protein misfolding and subsequent aggregation, thereby retarding the aggregation kinetics substantially. Graphical Abstract Cationic amphiphiles viz. N-methyl-8-(alkoxy)quinolinium iodide suppress the aggregation of HEWL. The control experiments show that the cationic head group as well as the aliphatic tail is indispensable for inhibition of aggregation process and removal of either head group or hydrocarbon chain obliterates its ability to do the same. Indian Academy of Sciences, 2013 Inhibitors nationallicence amyloid nationallicence protein nationallicence aggregation nationallicence conformation nationallicence HEWL nationallicence OJHA BIMLESH Department of Chemistry, Indian Institute of Technology Guwahati, 781 039, Assam, India aut KAR CHIRANTAN Department of Chemistry, Indian Institute of Technology Guwahati, 781 039, Assam, India aut DAS GOPAL Department of Chemistry, Indian Institute of Technology Guwahati, 781 039, Assam, India aut Journal of Chemical Sciences Springer-Verlag 125/2(2013-03-01), 229-236 0974-3626 125:2<229 2013 125 12039 https://doi.org/10.1007/s12039-013-0388-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12039-013-0388-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- OJHA BIMLESH Department of Chemistry, Indian Institute of Technology Guwahati, 781 039, Assam, India aut NATIONALLICENCE 700 1- KAR CHIRANTAN Department of Chemistry, Indian Institute of Technology Guwahati, 781 039, Assam, India aut NATIONALLICENCE 700 1- DAS GOPAL Department of Chemistry, Indian Institute of Technology Guwahati, 781 039, Assam, India aut NATIONALLICENCE 773 0- Journal of Chemical Sciences Springer-Verlag 125/2(2013-03-01), 229-236 0974-3626 125:2<229 2013 125 12039 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer