naa a22 4500 510737706 CHVBK 20180411083016.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s11284-013-1059-4 doi (NATIONALLICENCE)springer-10.1007/s11284-013-1059-4 Geographic association and temporal variation of chemical and physical defense and leaf damage in Datura stramonium [Elektronische Daten] [Guillermo Castillo, Laura Cruz, Johnattan Hernández-Cumplido, Ken Oyama, César Flores-Ortiz, Juan Fornoni, Pedro Valverde, Juan Núñez-Farfán] The evolution of plant defense traits has traditionally been explained trough the "coevolutionary arms race” between plants and herbivores. According to this, specialist herbivores have evolved to cope effectively with the defensive traits of their host plants and may even use them as a cue for host location. We analyzed the geographic association between leaf trichomes, two tropane alkaloids (putative resistance traits), and leaf damage by herbivores in 28 populations of Datura stramonium in central Mexico. Since the specialist leaf beetles Epitrix parvula and Lema trilineata are the main herbivores of D. stramonium in central Mexico, we predicted a positive association between plant defense and leaf damage across populations. Also, if physical environmental conditions (temperature or precipitation) constrain the expression of plant defense, then the geographic variation in leaf damage should be explained partially by the interaction between defensive traits and environmental factors. Furthermore, we studied the temporal and spatial variation in leaf trichome density and leaf damage in five selected populations of D. stramonium sampled in two periods (1997 vs. 2007). We found a positive association between leaf trichomes density and atropine concentration with leaf damage across populations. The interaction between defensive traits and water availability in each locality had a significant effect on the geographic variation in leaf damage. Differences among populations in leaf trichome density are maintained over time. Our results indicate that local plant-herbivore interaction plays an important role in shaping the geographic and temporal variation in plant defense in D. stramonium. The Ecological Society of Japan, 2013 Datura stramonium nationallicence Geographic variation nationallicence Herbivory nationallicence Tropane alkaloids nationallicence Leaf trichomes nationallicence Plant defense nationallicence Solanaceae nationallicence Castillo Guillermo Laboratorio de Genética Ecológica y Evolución, Instituto de Ecología, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, 04510, Mexico, Distrito Federal, Mexico aut Cruz Laura Laboratorio de Genética Ecológica y Evolución, Instituto de Ecología, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, 04510, Mexico, Distrito Federal, Mexico aut Hernández-Cumplido Johnattan Laboratory of Evolutionary Entomology, Institute of Biology, University of Neuchâtel (UNINE), Emile Argand 11, 2009, Neuchâtel, Switzerland aut Oyama Ken Centro de Investigaciones en Ecosistemas, Universidad Nacional Autónoma de México (UNAM), Antigua Carretera a Pátzcuaro 8701, Col. Ex-Hacienda de San José de la Huerta, 58190, Morelia, Michoacán, Mexico aut Flores-Ortiz César Laboratorio de Fisiología Vegetal, UBIPRO, Universidad Nacional Autónoma de México Facultad de Estudios Superiores Iztacala, Apartado Postal 314, 54090, Mexico, Estado de México, Mexico aut Fornoni Juan Laboratorio de Genética Ecológica y Evolución, Instituto de Ecología, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, 04510, Mexico, Distrito Federal, Mexico aut Valverde Pedro Departamento de Biología, Universidad Autónoma Metropolitana-Iztapalapa, Apartado postal 55-535, 09340, Mexico, Distrito Federal, Mexico aut Núñez-Farfán Juan Laboratorio de Genética Ecológica y Evolución, Instituto de Ecología, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, 04510, Mexico, Distrito Federal, Mexico aut Ecological Research Springer Japan 28/4(2013-07-01), 663-672 0912-3814 28:4<663 2013 28 11284 https://doi.org/10.1007/s11284-013-1059-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11284-013-1059-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Castillo Guillermo Laboratorio de Genética Ecológica y Evolución, Instituto de Ecología, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, 04510, Mexico, Distrito Federal, Mexico aut NATIONALLICENCE 700 1- Cruz Laura Laboratorio de Genética Ecológica y Evolución, Instituto de Ecología, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, 04510, Mexico, Distrito Federal, Mexico aut NATIONALLICENCE 700 1- Hernández-Cumplido Johnattan Laboratory of Evolutionary Entomology, Institute of Biology, University of Neuchâtel (UNINE), Emile Argand 11, 2009, Neuchâtel, Switzerland aut NATIONALLICENCE 700 1- Oyama Ken Centro de Investigaciones en Ecosistemas, Universidad Nacional Autónoma de México (UNAM), Antigua Carretera a Pátzcuaro 8701, Col. Ex-Hacienda de San José de la Huerta, 58190, Morelia, Michoacán, Mexico aut NATIONALLICENCE 700 1- Flores-Ortiz César Laboratorio de Fisiología Vegetal, UBIPRO, Universidad Nacional Autónoma de México Facultad de Estudios Superiores Iztacala, Apartado Postal 314, 54090, Mexico, Estado de México, Mexico aut NATIONALLICENCE 700 1- Fornoni Juan Laboratorio de Genética Ecológica y Evolución, Instituto de Ecología, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, 04510, Mexico, Distrito Federal, Mexico aut NATIONALLICENCE 700 1- Valverde Pedro Departamento de Biología, Universidad Autónoma Metropolitana-Iztapalapa, Apartado postal 55-535, 09340, Mexico, Distrito Federal, Mexico aut NATIONALLICENCE 700 1- Núñez-Farfán Juan Laboratorio de Genética Ecológica y Evolución, Instituto de Ecología, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, 04510, Mexico, Distrito Federal, Mexico aut NATIONALLICENCE 773 0- Ecological Research Springer Japan 28/4(2013-07-01), 663-672 0912-3814 28:4<663 2013 28 11284 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer