naa a22 4500 510738222 CHVBK 20180411083018.0 cr unu---uuuuu 180411e20130301xx s 000 0 eng 10.1007/s12127-013-0120-x doi (NATIONALLICENCE)springer-10.1007/s12127-013-0120-x Coupling electrospray corona discharge, charge reduction and ion mobility mass spectrometry: From peptides to large macromolecular protein complexes [Elektronische Daten] [Iain Campuzano, Paul Schnier] We present the design and implementation of a home-built point-to-plane corona discharge probe, which rapidly and efficiently charge reduces biological ions generated by electrospray ionization (ESI). The molecules analysed ranged from small peptides such as Glu-fibrinopeptide B (1.5kDa), small proteins such as myoglobin (16.9kDa), polymers such as polyethylene glycol (PEG 10k) which all showed intense singly charged ions; to large native multiprotein complexes such as GroEL (802kDa) which show a broad range of charge-reduced species. The corona discharge probe operates at atmospheric pressure and was directly interfaced with a standard-ESI or nanoflow-ESI source of quadrupole ion mobility time-of-flight mass spectrometer. The corona discharge probe is completely modular and could potentially be mounted to any commercial or research grade mass spectrometer with an ESI source. The level of charge reduction is precisely controlled by the applied voltage and/or probe gas flow rate and when in operation, results in approximately a 50% reduction in total ion current. We also present the combination of corona discharge and travelling wave ion mobility and assign helium collision cross-section values (ΩHe) to the charge reduced species of the native protein complex pyruvate kinase. It would appear that the ΩHe of the +20 charge state for pyruvate kinase is approximately 20% smaller than the +35 charge state. Finally, we discuss the potential benefits and concerns of utilising charge reduced protein species as a means of extending the travelling wave collision cross-section calibration range over that which is already published. Springer-Verlag Berlin Heidelberg, 2013 Corona discharge nationallicence Electrospray ionization nationallicence Travelling wave ion mobility nationallicence Peptide nationallicence Polyethylene glycol and native protein complex nationallicence Campuzano Iain Department of Molecular Structure and Characterization, Amgen, 91320, Thousand Oaks, CA, USA aut Schnier Paul Department of Molecular Structure and Characterization, Amgen, 91320, Thousand Oaks, CA, USA aut International Journal for Ion Mobility Spectrometry Springer-Verlag 16/1(2013-03-01), 51-60 1435-6163 16:1<51 2013 16 12127 https://doi.org/10.1007/s12127-013-0120-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12127-013-0120-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Campuzano Iain Department of Molecular Structure and Characterization, Amgen, 91320, Thousand Oaks, CA, USA aut NATIONALLICENCE 700 1- Schnier Paul Department of Molecular Structure and Characterization, Amgen, 91320, Thousand Oaks, CA, USA aut NATIONALLICENCE 773 0- International Journal for Ion Mobility Spectrometry Springer-Verlag 16/1(2013-03-01), 51-60 1435-6163 16:1<51 2013 16 12127 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer