naa a22 4500 510738370 CHVBK 20180411083019.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s12127-012-0102-4 doi (NATIONALLICENCE)springer-10.1007/s12127-012-0102-4 Monitoring dynamic changes in lymph metabolome of fasting and fed rats by matrix-assisted laser desorption/ionization-ion mobility mass spectrometry (MALDI-IMMS) [Elektronische Daten] [Kimberly Kaplan, Shelley Jackson, Prabha Dwivedi, W. Davidson, Qing Yang, Patrick Tso, William Siems, Amina Woods, Herbert Hill Jr.] Matrix-assisted laser/desorption ionization (MALDI) time-of-flight mass spectrometry (TOF) has been investigated for use in the field of metabolomics; however, difficulties, mainly due to chemical interferences, are typically encountered. By coupling MALDI with ion mobility time-of-flight mass spectrometry (IMMS), isomers and isobars are resolved in mobility space reducing the chemical interference from matrix/background ions. MALDI-IMMS offers the advantages of high sensitivity, high throughput and low sample consumption. For this study, MALDI-IMMS was evaluated by monitoring metabolic changes in lymphatic fluid collected from fasting and fed rats. The number of metabolite features detected in the samples ranged between 1200 and 3400 depending on the duration between the feeding time and lymph sample collection. There were 747 metabolite features that were statistically analyzed by principal component analysis (PCA). From the 3-D score plots of PC1, PC2 and PC3 65% of the original variation of the system was explained and the differences between the samples were demonstrated. Springer-Verlag, 2012 MALDI-IMMS nationallicence Metabolomics nationallicence Principal component analysis nationallicence Kaplan Kimberly Washington State University, 99164, Pullman, WA, USA aut Jackson Shelley NIDA IRP, NIH, 21224, Baltimore, MD, USA aut Dwivedi Prabha Washington State University, 99164, Pullman, WA, USA aut Davidson W. University of Cincinnati, 45221, Cincinnati, OH, USA aut Yang Qing University of Cincinnati, 45221, Cincinnati, OH, USA aut Tso Patrick University of Cincinnati, 45221, Cincinnati, OH, USA aut Siems William Washington State University, 99164, Pullman, WA, USA aut Woods Amina NIDA IRP, NIH, 21224, Baltimore, MD, USA aut Hill Jr. Herbert Washington State University, 99164, Pullman, WA, USA aut International Journal for Ion Mobility Spectrometry Springer Berlin Heidelberg 16/3(2013-09-01), 177-184 1435-6163 16:3<177 2013 16 12127 https://doi.org/10.1007/s12127-012-0102-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12127-012-0102-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kaplan Kimberly Washington State University, 99164, Pullman, WA, USA aut NATIONALLICENCE 700 1- Jackson Shelley NIDA IRP, NIH, 21224, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Dwivedi Prabha Washington State University, 99164, Pullman, WA, USA aut NATIONALLICENCE 700 1- Davidson W. University of Cincinnati, 45221, Cincinnati, OH, USA aut NATIONALLICENCE 700 1- Yang Qing University of Cincinnati, 45221, Cincinnati, OH, USA aut NATIONALLICENCE 700 1- Tso Patrick University of Cincinnati, 45221, Cincinnati, OH, USA aut NATIONALLICENCE 700 1- Siems William Washington State University, 99164, Pullman, WA, USA aut NATIONALLICENCE 700 1- Woods Amina NIDA IRP, NIH, 21224, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Hill Jr Herbert Washington State University, 99164, Pullman, WA, USA aut NATIONALLICENCE 773 0- International Journal for Ion Mobility Spectrometry Springer Berlin Heidelberg 16/3(2013-09-01), 177-184 1435-6163 16:3<177 2013 16 12127 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer