naa a22 4500 510738443 CHVBK 20180411083019.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s12127-013-0124-6 doi (NATIONALLICENCE)springer-10.1007/s12127-013-0124-6 Matrix assisted laser desorption ionisation ion mobility separation mass spectrometry imaging of ex-vivo human skin [Elektronische Daten] [Philippa Hart, Simona Francese, M. Woodroofe, Malcolm Clench] Matrix assisted laser desorption ionisation ion mobility separation mass spectrometry imaging (MALDI-IMS-MSI) has been employed to image the distribution of proteins in ex-vivo human skin. Using a "bottom-up” proteomics approach employing "on-tissue” digestion the distribution of abundant skin proteins; collagen, keratin, decorin and serum albumin could be mapped. Images have been recorded at 150 and 30μm spatial resolution. Multivariate statistical analysis of the data has been employed to associate specific proteins with layers of the skin. The improved specificity given by the use of ion mobility separation in mass spectrometric imaging has been demonstrated by separation of peptide ions from phospholipids. Springer-Verlag Berlin Heidelberg, 2013 MALDI nationallicence Skin nationallicence MALDI-imaging nationallicence Peptides nationallicence Hart Philippa Biomedical Research Centre, Sheffield Hallam University, Howard Street, S11WB, Sheffield, UK aut Francese Simona Biomedical Research Centre, Sheffield Hallam University, Howard Street, S11WB, Sheffield, UK aut Woodroofe M. Biomedical Research Centre, Sheffield Hallam University, Howard Street, S11WB, Sheffield, UK aut Clench Malcolm Biomedical Research Centre, Sheffield Hallam University, Howard Street, S11WB, Sheffield, UK aut International Journal for Ion Mobility Spectrometry Springer-Verlag 16/2(2013-06-01), 71-83 1435-6163 16:2<71 2013 16 12127 https://doi.org/10.1007/s12127-013-0124-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12127-013-0124-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hart Philippa Biomedical Research Centre, Sheffield Hallam University, Howard Street, S11WB, Sheffield, UK aut NATIONALLICENCE 700 1- Francese Simona Biomedical Research Centre, Sheffield Hallam University, Howard Street, S11WB, Sheffield, UK aut NATIONALLICENCE 700 1- Woodroofe M. Biomedical Research Centre, Sheffield Hallam University, Howard Street, S11WB, Sheffield, UK aut NATIONALLICENCE 700 1- Clench Malcolm Biomedical Research Centre, Sheffield Hallam University, Howard Street, S11WB, Sheffield, UK aut NATIONALLICENCE 773 0- International Journal for Ion Mobility Spectrometry Springer-Verlag 16/2(2013-06-01), 71-83 1435-6163 16:2<71 2013 16 12127 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer