naa a22 4500 510738931 CHVBK 20180411083021.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s12015-012-9380-9 doi (NATIONALLICENCE)springer-10.1007/s12015-012-9380-9 Systemic Delivery of Human Mesenchymal Stromal Cells Combined with IGF-1 Enhances Muscle Functional Recovery in LAMA2 dy/2j Dystrophic Mice [Elektronische Daten] [Mariane Secco, Carlos Bueno Jr, Natassia Vieira, Camila Almeida, Mayra Pelatti, Eder Zucconi, Paolo Bartolini, Mariz Vainzof, Elen Miyabara, Oswaldo Okamoto, Mayana Zatz] The combination of cell therapy with growth factors could be a useful approach to treat progressive muscular dystrophies. Here, we demonstrate, for the first time, that IGF-1 considerably enhances the myogenesis of human umbilical cord (UC) mesenchymal stromal cells (MSCs) in vitro and that IGF-1 enhances interaction and restoration of dystrophin expression in co-cultures of MSCs and muscle cells from Duchenne patients. In vivo studies showed that human MSCs were able to reach the skeletal muscle of LAMA2 dy/2j dystrophic mice, through systemic delivery, without immunosuppression. Moreover, we showed, for the first time, that IGF-1 injected systemically together with MSCs markedly reduced muscle inflammation and fibrosis, and significantly improved muscle strength in dystrophic mice. Our results suggest that a combined treatment with IGF-1 and MSCs enhances efficiency of muscle repair and, therefore, should be further considered as a potential therapeutic approach in muscular dystrophies. Springer Science+Business Media, LLC, 2012 Muscular dystrophies nationallicence IGF-1 nationallicence Mesenchymal stromal cells nationallicence Secco Mariane Human Genome Research Center, Department of Genetic and Evolutionary Biology, Institute of Biosciences, São Paulo, Brazil aut Bueno Jr Carlos Human Genome Research Center, Department of Genetic and Evolutionary Biology, Institute of Biosciences, São Paulo, Brazil aut Vieira Natassia Human Genome Research Center, Department of Genetic and Evolutionary Biology, Institute of Biosciences, São Paulo, Brazil aut Almeida Camila Human Genome Research Center, Department of Genetic and Evolutionary Biology, Institute of Biosciences, São Paulo, Brazil aut Pelatti Mayra Human Genome Research Center, Department of Genetic and Evolutionary Biology, Institute of Biosciences, São Paulo, Brazil aut Zucconi Eder Human Genome Research Center, Department of Genetic and Evolutionary Biology, Institute of Biosciences, São Paulo, Brazil aut Bartolini Paolo Department of Biotechnology, National Nuclear Energy Commission-IPEN-CNEN, São Paulo, Brazil aut Vainzof Mariz Human Genome Research Center, Department of Genetic and Evolutionary Biology, Institute of Biosciences, São Paulo, Brazil aut Miyabara Elen Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil aut Okamoto Oswaldo Human Genome Research Center, Department of Genetic and Evolutionary Biology, Institute of Biosciences, São Paulo, Brazil aut Zatz Mayana Human Genome Research Center, Department of Genetic and Evolutionary Biology, Institute of Biosciences, São Paulo, Brazil aut Stem Cell Reviews and Reports Humana Press Inc 9/1(2013-02-01), 93-109 1550-8943 9:1<93 2013 9 12015 https://doi.org/10.1007/s12015-012-9380-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-012-9380-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Secco Mariane Human Genome Research Center, Department of Genetic and Evolutionary Biology, Institute of Biosciences, São Paulo, Brazil aut NATIONALLICENCE 700 1- Bueno Jr Carlos Human Genome Research Center, Department of Genetic and Evolutionary Biology, Institute of Biosciences, São Paulo, Brazil aut NATIONALLICENCE 700 1- Vieira Natassia Human Genome Research Center, Department of Genetic and Evolutionary Biology, Institute of Biosciences, São Paulo, Brazil aut NATIONALLICENCE 700 1- Almeida Camila Human Genome Research Center, Department of Genetic and Evolutionary Biology, Institute of Biosciences, São Paulo, Brazil aut NATIONALLICENCE 700 1- Pelatti Mayra Human Genome Research Center, Department of Genetic and Evolutionary Biology, Institute of Biosciences, São Paulo, Brazil aut NATIONALLICENCE 700 1- Zucconi Eder Human Genome Research Center, Department of Genetic and Evolutionary Biology, Institute of Biosciences, São Paulo, Brazil aut NATIONALLICENCE 700 1- Bartolini Paolo Department of Biotechnology, National Nuclear Energy Commission-IPEN-CNEN, São Paulo, Brazil aut NATIONALLICENCE 700 1- Vainzof Mariz Human Genome Research Center, Department of Genetic and Evolutionary Biology, Institute of Biosciences, São Paulo, Brazil aut NATIONALLICENCE 700 1- Miyabara Elen Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil aut NATIONALLICENCE 700 1- Okamoto Oswaldo Human Genome Research Center, Department of Genetic and Evolutionary Biology, Institute of Biosciences, São Paulo, Brazil aut NATIONALLICENCE 700 1- Zatz Mayana Human Genome Research Center, Department of Genetic and Evolutionary Biology, Institute of Biosciences, São Paulo, Brazil aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Humana Press Inc 9/1(2013-02-01), 93-109 1550-8943 9:1<93 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer