naa a22 4500 510738966 CHVBK 20180411083021.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s12015-012-9385-4 doi (NATIONALLICENCE)springer-10.1007/s12015-012-9385-4 Phenotypic and Functional Characterization of Mesenchymal Stem Cells from Chorionic Villi of Human Term Placenta [Elektronische Daten] [M. Abumaree, M. Al Jumah, B. Kalionis, D. Jawdat, A. Al Khaldi, A. AlTalabani, B. Knawy] Background: Bone marrow derived mesenchymal stem cells (BM-MSCs) are used extensively in transplantation but their use is associated with many problems including low abundance in BM, low overall number, decreased differentiation potential with age and the invasive isolation procedures needed to obtain BM. We report a novel method of isolating placental MSCs (pMSCs) from chorionic villi, which exhibit the phenotypic and functional characteristics that will make them an attractive source of MSCs for cell-based therapy. Methods: A novel explant approach was used to isolate pMSCs from chorionic villi of human placentae. These pMSCs were characterized by flow cytometry and were differentiated into adipocytes, osteocytes and chondrocytes using differentiation medium as demonstrated by cytochemical staining. The gene and protein expression profiles of pMSCs were also characterized using real time polymerase chain reaction (PCR) and flow cytometry, respectively. In addition, cytokine secretion by pMSCs was also analysed using sandwich enzyme-linked immunosorbent assay (ELISA) technique. Moreover, the migration and proliferation potentials of pMSCs were also determined. Results: pMSCs were isolated from fetal part of the chorionic villi and these pMSCs expressed CD44, CD90, CD105, CD146, CD166 and HLA-ABC but not CD14, CD19, CD40, CD45, CD80, CD83, CD86 and HLA-DR. In addition, these pMSCs differentiated into osteocytes, chondrocytes and adipocytes and they also expressed several adhesion molecules, chemokines/receptors, growth factor receptors and cytokines/receptors. Moreover, they secreted many cytokines (IL-1Ra, IL6, IL8, IL10, IL11 and IL15) and they were able to proliferate. Furthermore, they migrated in response to chemotactic factors including stromal cell-derived factor-1 (SDF-1), platelet derived growth factor (PDGF), hepatocyte growth factor (HGF), and monocyte chemotactic protein-1 (MCP-1). Conclusions: We devised a novel explant method of isolating pMSCs that expressed many biological factors responsible for mediating cellular processes such as migration/homing, immune modulation and angiogenesis. Therefore, we suggest that pMSCs prepared from human term placental chorionic villous explants are an attractive source of MSCs for cell therapy. Springer Science+Business Media, LLC, 2012 Chorionic villi nationallicence Placental mesenchymal stem cells nationallicence Characterization nationallicence Migration nationallicence Proliferation nationallicence Abumaree M. College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, King Abdulaziz Medical City, National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia aut Al Jumah M. College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, King Abdulaziz Medical City, National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia aut Kalionis B. Department of Obstetrics and Gynaecology, University of Melbourne, 3052, Parkville, Victoria, Australia aut Jawdat D. College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, King Abdulaziz Medical City, National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia aut Al Khaldi A. College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, King Abdulaziz Medical City, National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia aut AlTalabani A. College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, King Abdulaziz Medical City, National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia aut Knawy B. College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, King Abdulaziz Medical City, National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia aut Stem Cell Reviews and Reports Humana Press Inc 9/1(2013-02-01), 16-31 1550-8943 9:1<16 2013 9 12015 https://doi.org/10.1007/s12015-012-9385-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-012-9385-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Abumaree M. College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, King Abdulaziz Medical City, National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia aut NATIONALLICENCE 700 1- Al Jumah M. College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, King Abdulaziz Medical City, National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia aut NATIONALLICENCE 700 1- Kalionis B. Department of Obstetrics and Gynaecology, University of Melbourne, 3052, Parkville, Victoria, Australia aut NATIONALLICENCE 700 1- Jawdat D. College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, King Abdulaziz Medical City, National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia aut NATIONALLICENCE 700 1- Al Khaldi A. College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, King Abdulaziz Medical City, National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia aut NATIONALLICENCE 700 1- AlTalabani A. College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, King Abdulaziz Medical City, National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia aut NATIONALLICENCE 700 1- Knawy B. College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, King Abdulaziz Medical City, National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Humana Press Inc 9/1(2013-02-01), 16-31 1550-8943 9:1<16 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer