naa a22 4500 510738974 CHVBK 20180411083021.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s12015-012-9392-5 doi (NATIONALLICENCE)springer-10.1007/s12015-012-9392-5 Transcriptional Analysis of Histone Deacetylase Family Members Reveal Similarities Between Differentiating and Aging Spermatogonial Stem Cells [Elektronische Daten] [Amber Kofman, Jessica Huszar, Christopher Payne] The differentiation of adult stem cells involves extensive chromatin remodeling, mediated in part by the gene products of histone deacetylase (HDAC) family members. While the transcriptional downregulation of HDACs can impede stem cell self-renewal in certain contexts, it may also promote stem cell maintenance under other circumstances. In self-renewing, differentiating, and aging spermatogonial stem cells (SSCs), the gene expression dynamics of HDACs have not yet been characterized. To gain further insight with these studies, we analyzed the transcriptional profiles of six HDAC family members, previously identified to be the most highly expressed in self-renewing SSCs, during stem cell differentiation and aging. Here we discovered that in both differentiating and aging SSCs the expression of Sirt4 increases, while the expression of Hdac2, Hdac6, and Sirt1 decreases. When SSCs are exposed to the lifespan-enhancing drug rapamycin in vivo, the resultant HDAC gene expression patterns are opposite of those seen in the differentiating and aging SSCs, with increased Hdac2, Hdac6, and Sirt1 and decreased Hdac8, Hdac9, and Sirt4. Our findings suggest that HDACs important for stem cell maintenance and oxidative capacity are downregulated as adult stem cells differentiate or age. These results provide important insights into the epigenetic regulation of stem cell differentiation and aging in mammals. Springer Science+Business Media, LLC, 2012 Histone deacetylase nationallicence Stem cell nationallicence Spermatogonia nationallicence Differentiation nationallicence Aging nationallicence Sirtuin nationallicence Gene expression nationallicence Kofman Amber Human Molecular Genetics Program, Children's Memorial Research Center, 60614, Chicago, IL, USA aut Huszar Jessica Human Molecular Genetics Program, Children's Memorial Research Center, 60614, Chicago, IL, USA aut Payne Christopher Human Molecular Genetics Program, Children's Memorial Research Center, 60614, Chicago, IL, USA aut Stem Cell Reviews and Reports Humana Press Inc 9/1(2013-02-01), 59-64 1550-8943 9:1<59 2013 9 12015 https://doi.org/10.1007/s12015-012-9392-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-012-9392-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kofman Amber Human Molecular Genetics Program, Children's Memorial Research Center, 60614, Chicago, IL, USA aut NATIONALLICENCE 700 1- Huszar Jessica Human Molecular Genetics Program, Children's Memorial Research Center, 60614, Chicago, IL, USA aut NATIONALLICENCE 700 1- Payne Christopher Human Molecular Genetics Program, Children's Memorial Research Center, 60614, Chicago, IL, USA aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Humana Press Inc 9/1(2013-02-01), 59-64 1550-8943 9:1<59 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer