naa a22 4500 510738990 CHVBK 20180411083021.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s12015-012-9365-8 doi (NATIONALLICENCE)springer-10.1007/s12015-012-9365-8 Human Stromal (Mesenchymal) Stem Cells from Bone Marrow, Adipose Tissue and Skin Exhibit Differences in Molecular Phenotype and Differentiation Potential [Elektronische Daten] [May Al-Nbaheen, Radhakrishnan vishnubalaji, Dalia Ali, Amel Bouslimi, Fawzi Al-Jassir, Matthias Megges, Alessandro Prigione, James Adjaye, Moustapha Kassem, Abdullah Aldahmash] Human stromal (mesenchymal) stem cells (hMSCs) are multipotent stem cells with ability to differentiate into mesoderm-type cells e.g. osteoblasts and adipocytes and thus they are being introduced into clinical trials for tissue regeneration. Traditionally, hMSCs have been isolated from bone marrow, but the number of cells obtained is limited. Here, we compared the MSC-like cell populations, obtained from alternative sources for MSC: adipose tissue and skin, with the standard phenotype of human bone marrow MSC (BM-MSCs). MSC from human adipose tissue (human adipose stromal cells (hATSCs)) and human skin (human adult skin stromal cells, (hASSCs) and human new-born skin stromal cells (hNSSCs)) grew readily in culture and the growth rate was highest in hNSSCs and lowest in hATSCs. Compared with phenotype of hBM-MSC, all cell populations were CD34−, CD45−, CD14−, CD31−, HLA-DR−, CD13+, CD29+, CD44+, CD73+, CD90+,and CD105+. When exposed to in vitro differentiation, hATSCs, hASSCs and hNSSCs exhibited quantitative differences in their ability to differentiate into adipocytes and to osteoblastic cells. Using a microarray-based approach we have unveiled a common MSC molecular signature composed of 33 CD markers including known MSC markers and several novel markers e.g. CD165, CD276, and CD82. However, significant differences in the molecular phenotype between these different stromal cell populations were observed suggesting ontological and functional differences. In conclusion, MSC populations obtained from different tissues exhibit significant differences in their proliferation, differentiation and molecular phenotype, which should be taken into consideration when planning their use in clinical protocols. The Author(s), 2012 Stromal cells nationallicence Mesenchymal stem cell nationallicence Adipose tissue nationallicence Bone marrow nationallicence Skin nationallicence DNA microarray nationallicence Al-Nbaheen May Stem Cell Unit, Department of Anatomy 28, College of Medicine, King Saud University, P.O. Box 2925, 11461, Riyadh, Kingdom of Saudi Arabia aut vishnubalaji Radhakrishnan Stem Cell Unit, Department of Anatomy 28, College of Medicine, King Saud University, P.O. Box 2925, 11461, Riyadh, Kingdom of Saudi Arabia aut Ali Dalia Stem Cell Unit, Department of Anatomy 28, College of Medicine, King Saud University, P.O. Box 2925, 11461, Riyadh, Kingdom of Saudi Arabia aut Bouslimi Amel Stem Cell Unit, Department of Anatomy 28, College of Medicine, King Saud University, P.O. Box 2925, 11461, Riyadh, Kingdom of Saudi Arabia aut Al-Jassir Fawzi Department of Orthopedic Surgery, King Khalid University Hospital, College of Medicine, King Saud University, Riyadh, Kingdom of Saudi Arabia aut Megges Matthias Department of Vertebrate Genomics, Molecular Embryology and Aging group, Max Planck Institute for Molecular Genetics, Ihnestr. 63-73, 14195, Berlin, Germany aut Prigione Alessandro Department of Vertebrate Genomics, Molecular Embryology and Aging group, Max Planck Institute for Molecular Genetics, Ihnestr. 63-73, 14195, Berlin, Germany aut Adjaye James Stem Cell Unit, Department of Anatomy 28, College of Medicine, King Saud University, P.O. Box 2925, 11461, Riyadh, Kingdom of Saudi Arabia aut Kassem Moustapha Stem Cell Unit, Department of Anatomy 28, College of Medicine, King Saud University, P.O. Box 2925, 11461, Riyadh, Kingdom of Saudi Arabia aut Aldahmash Abdullah Stem Cell Unit, Department of Anatomy 28, College of Medicine, King Saud University, P.O. Box 2925, 11461, Riyadh, Kingdom of Saudi Arabia aut Stem Cell Reviews and Reports Humana Press Inc 9/1(2013-02-01), 32-43 1550-8943 9:1<32 2013 9 12015 https://doi.org/10.1007/s12015-012-9365-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-012-9365-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Al-Nbaheen May Stem Cell Unit, Department of Anatomy 28, College of Medicine, King Saud University, P.O. Box 2925, 11461, Riyadh, Kingdom of Saudi Arabia aut NATIONALLICENCE 700 1- vishnubalaji Radhakrishnan Stem Cell Unit, Department of Anatomy 28, College of Medicine, King Saud University, P.O. Box 2925, 11461, Riyadh, Kingdom of Saudi Arabia aut NATIONALLICENCE 700 1- Ali Dalia Stem Cell Unit, Department of Anatomy 28, College of Medicine, King Saud University, P.O. Box 2925, 11461, Riyadh, Kingdom of Saudi Arabia aut NATIONALLICENCE 700 1- Bouslimi Amel Stem Cell Unit, Department of Anatomy 28, College of Medicine, King Saud University, P.O. Box 2925, 11461, Riyadh, Kingdom of Saudi Arabia aut NATIONALLICENCE 700 1- Al-Jassir Fawzi Department of Orthopedic Surgery, King Khalid University Hospital, College of Medicine, King Saud University, Riyadh, Kingdom of Saudi Arabia aut NATIONALLICENCE 700 1- Megges Matthias Department of Vertebrate Genomics, Molecular Embryology and Aging group, Max Planck Institute for Molecular Genetics, Ihnestr. 63-73, 14195, Berlin, Germany aut NATIONALLICENCE 700 1- Prigione Alessandro Department of Vertebrate Genomics, Molecular Embryology and Aging group, Max Planck Institute for Molecular Genetics, Ihnestr. 63-73, 14195, Berlin, Germany aut NATIONALLICENCE 700 1- Adjaye James Stem Cell Unit, Department of Anatomy 28, College of Medicine, King Saud University, P.O. Box 2925, 11461, Riyadh, Kingdom of Saudi Arabia aut NATIONALLICENCE 700 1- Kassem Moustapha Stem Cell Unit, Department of Anatomy 28, College of Medicine, King Saud University, P.O. Box 2925, 11461, Riyadh, Kingdom of Saudi Arabia aut NATIONALLICENCE 700 1- Aldahmash Abdullah Stem Cell Unit, Department of Anatomy 28, College of Medicine, King Saud University, P.O. Box 2925, 11461, Riyadh, Kingdom of Saudi Arabia aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Humana Press Inc 9/1(2013-02-01), 32-43 1550-8943 9:1<32 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer