naa a22 4500 510739008 CHVBK 20180411083021.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s12015-012-9395-2 doi (NATIONALLICENCE)springer-10.1007/s12015-012-9395-2 Mesenchymal Stem Cell: Does it Work in an Experimental Model with Acute Respiratory Distress Syndrome? [Elektronische Daten] [Sema Yilmaz, Nihal Inandiklioglu, Dincer Yildizdas, Cansu Subasi, Arbil Acikalin, Yurdun Kuyucu, Ibrahim Bayram, Ali Topak, Atila Tanyeli, Gokhan Duruksu, Erdal Karaoz] We hypothesized that bone marrow-derived mesenchymal stem cells (BM-MSCs) would have a possible role in the treatment of acute respiratory distress syndrome (ARDS). ARDS disease model was developed in Wistar albino male rats by intratracheal instillation of physiological saline solution. Anesthezied and tracheotomized rats (n = 8) with ARDS were pressure-controlled ventilated. Isolated and characterized rat (r-) BM-MSCs were labeled with GFP gene, and introduced in the lungs of the ARDS rat-model. After applying of MSCs, the life span of each rat was recorded. When rats died, their lung tissues were removed for histopathological examination. Also the tissue sections were analyzed for GFP labeled rBM-MSCs and stained for vimentin, CK19, proinflammatory (MPO, IL-1β, IL-6 and MIP-2) and anti-inflammatory [IL-1ra and prostaglandin E2 receptor (EP3)] cytokines. The histopathological signs of rat-model ARDS were similar to the acute phase of ARDS in humans. rBM-MSCs were observed to home in lung paranchyma. Although the infiltration of neutrophils slightly decreased in the interalveolar, peribronchial and perivascular area, a notable improvement was determined in the degree of hemorrhage, edema and hyaline membrane formation in rats treated with rBM-MSCs. Also decreased proinflammatory cytokines levels and increased the intensity of anti-inflammatory cytokines were established. Therefore MSCs could promote alveoar epithelial repair by mediating of cytokines from a proinflammatory to an anti-inflammatory response. As a novel therapeutic approach, mesenchymal stem cell treatment with intratracheal injection could be helpful in the management of critically ill patients with ARDS. Springer Science+Business Media, LLC, 2012 Acute respiratory distress syndrome nationallicence Mesenchymal stem cell nationallicence Cytokine nationallicence Inflammation nationallicence Critically ill nationallicence Yilmaz Sema Department of Pediatric Hematology/Oncology, Ondokuz Mayıs University Faculty of Medicine, 55220, Atakum, Samsun, Turkey aut Inandiklioglu Nihal Department of Medical Biology, Cukurova University Faculty of Medicine, Adana, Turkey aut Yildizdas Dincer Department of Pediatric Intensive Care Unit, Cukurova University Faculty of Medicine, Adana, Turkey aut Subasi Cansu Institute of Health Sciences, Stem Cell Department, Kocaeli University Center for Stem Cell and Gene Therapies Research and Practice, 41380, Umuttepe-İzmit, Kocaeli, Turkey aut Acikalin Arbil Department of Pathology, Cukurova University Medical Faculty, Adana, Turkey aut Kuyucu Yurdun Department of Histology, Cukurova University Faculty of Medicine, Adana, Turkey aut Bayram Ibrahim Department of Pediatric Hematology/Oncology, Cukurova University Faculty of Medicine, Adana, Turkey aut Topak Ali Department of Experimental Research and Practice Center of Medical, Cukurova University Faculty of Medicine, Adana, Turkey aut Tanyeli Atila Department of Pediatric Hematology/Oncology, Cukurova University Faculty of Medicine, Adana, Turkey aut Duruksu Gokhan Institute fo Health Sciences,Stem Cell Department, Kocaeli University Center for Stem Cell and Gene Therapies research and Practice, Adana, Turkey aut Karaoz Erdal Institute of Health Sciences, Stem Cell Department, Kocaeli University Center for Stem Cell and Gene Therapies Research and Practice, 41380, Umuttepe-İzmit, Kocaeli, Turkey aut Stem Cell Reviews and Reports Humana Press Inc 9/1(2013-02-01), 80-92 1550-8943 9:1<80 2013 9 12015 https://doi.org/10.1007/s12015-012-9395-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-012-9395-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yilmaz Sema Department of Pediatric Hematology/Oncology, Ondokuz Mayıs University Faculty of Medicine, 55220, Atakum, Samsun, Turkey aut NATIONALLICENCE 700 1- Inandiklioglu Nihal Department of Medical Biology, Cukurova University Faculty of Medicine, Adana, Turkey aut NATIONALLICENCE 700 1- Yildizdas Dincer Department of Pediatric Intensive Care Unit, Cukurova University Faculty of Medicine, Adana, Turkey aut NATIONALLICENCE 700 1- Subasi Cansu Institute of Health Sciences, Stem Cell Department, Kocaeli University Center for Stem Cell and Gene Therapies Research and Practice, 41380, Umuttepe-İzmit, Kocaeli, Turkey aut NATIONALLICENCE 700 1- Acikalin Arbil Department of Pathology, Cukurova University Medical Faculty, Adana, Turkey aut NATIONALLICENCE 700 1- Kuyucu Yurdun Department of Histology, Cukurova University Faculty of Medicine, Adana, Turkey aut NATIONALLICENCE 700 1- Bayram Ibrahim Department of Pediatric Hematology/Oncology, Cukurova University Faculty of Medicine, Adana, Turkey aut NATIONALLICENCE 700 1- Topak Ali Department of Experimental Research and Practice Center of Medical, Cukurova University Faculty of Medicine, Adana, Turkey aut NATIONALLICENCE 700 1- Tanyeli Atila Department of Pediatric Hematology/Oncology, Cukurova University Faculty of Medicine, Adana, Turkey aut NATIONALLICENCE 700 1- Duruksu Gokhan Institute fo Health Sciences,Stem Cell Department, Kocaeli University Center for Stem Cell and Gene Therapies research and Practice, Adana, Turkey aut NATIONALLICENCE 700 1- Karaoz Erdal Institute of Health Sciences, Stem Cell Department, Kocaeli University Center for Stem Cell and Gene Therapies Research and Practice, 41380, Umuttepe-İzmit, Kocaeli, Turkey aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Humana Press Inc 9/1(2013-02-01), 80-92 1550-8943 9:1<80 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer