naa a22 4500 510739024 CHVBK 20180411083021.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s12015-013-9457-0 doi (NATIONALLICENCE)springer-10.1007/s12015-013-9457-0 Generating Hypoimmunogenic Human Embryonic Stem Cells by the Disruption of Beta 2-Microglobulin [Elektronische Daten] [Pengfei Lu, Jijun Chen, Lixiazi He, Jiangtao Ren, Haide Chen, Lingjun Rao, Qinggang Zhuang, Hui Li, Lei Li, Lei Bao, Ji He, Wei Zhang, Faming Zhu, Chun Cui, Lei Xiao] Immune rejection hinders the application of human embryonic stem cells (hESCs) in transplantation therapy. Human leukocyte antigens (HLAs) on the cell surface are the major cause of graft rejection. In this study, we generated HLA class I-deficient hESCs via disruption of beta 2-microglobulin (β2m), the light chain of HLA Class I. We found that HLA class I proteins were not present on the cell surface of β2m-null hESCs. These cells showed the same pluripotency as wildtype hESCs and demonstrated hypoimmunogenicity. Thus, HLA class I-deficient hESCs might serve as an unlimited cell source for the generation of universally compatible "off-the-shelf” cell grafts, tissues or organs in the future. Springer Science+Business Media New York, 2013 Beta 2-microglobulin nationallicence Human leukocyte antigens nationallicence Hypoimmunogenicity nationallicence Human embryonic stem cells nationallicence Immune rejection nationallicence iPS cells nationallicence Lu Pengfei Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, Shanghai, People's Republic of China aut Chen Jijun Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, Shanghai, People's Republic of China aut He Lixiazi Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, Shanghai, People's Republic of China aut Ren Jiangtao Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, Shanghai, People's Republic of China aut Chen Haide Zhejiang University School of Medicine, 866 Yuhangtang Road, Nongshenghuan Building E#, Room 301, 310058, Hangzhou, People's Republic of China aut Rao Lingjun Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, Shanghai, People's Republic of China aut Zhuang Qinggang Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, Shanghai, People's Republic of China aut Li Hui Xiangtan Center Hospital, 411100, Hunan, People's Republic of China aut Li Lei Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, Shanghai, People's Republic of China aut Bao Lei Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, Shanghai, People's Republic of China aut He Ji Blood Centre of Zhejiang Province, 310058, Hangzhou, People's Republic of China aut Zhang Wei Blood Centre of Zhejiang Province, 310058, Hangzhou, People's Republic of China aut Zhu Faming Blood Centre of Zhejiang Province, 310058, Hangzhou, People's Republic of China aut Cui Chun Zhejiang University School of Medicine, 866 Yuhangtang Road, Nongshenghuan Building E#, Room 301, 310058, Hangzhou, People's Republic of China aut Xiao Lei Zhejiang University School of Medicine, 866 Yuhangtang Road, Nongshenghuan Building E#, Room 301, 310058, Hangzhou, People's Republic of China aut Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/6(2013-12-01), 806-813 1550-8943 9:6<806 2013 9 12015 https://doi.org/10.1007/s12015-013-9457-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-013-9457-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lu Pengfei Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Chen Jijun Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- He Lixiazi Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Ren Jiangtao Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Chen Haide Zhejiang University School of Medicine, 866 Yuhangtang Road, Nongshenghuan Building E#, Room 301, 310058, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Rao Lingjun Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhuang Qinggang Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Li Hui Xiangtan Center Hospital, 411100, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Li Lei Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Bao Lei Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- He Ji Blood Centre of Zhejiang Province, 310058, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Wei Blood Centre of Zhejiang Province, 310058, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Faming Blood Centre of Zhejiang Province, 310058, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Cui Chun Zhejiang University School of Medicine, 866 Yuhangtang Road, Nongshenghuan Building E#, Room 301, 310058, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Xiao Lei Zhejiang University School of Medicine, 866 Yuhangtang Road, Nongshenghuan Building E#, Room 301, 310058, Hangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/6(2013-12-01), 806-813 1550-8943 9:6<806 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer