naa a22 4500 510739180 CHVBK 20180411083022.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s12015-012-9366-7 doi (NATIONALLICENCE)springer-10.1007/s12015-012-9366-7 Optimization of the Cardiovascular Therapeutic Properties of Mesenchymal Stromal/Stem Cells-Taking the Next Step [Elektronische Daten] [James Richardson, Adam Nelson, Andrew Zannettino, Stan Gronthos, Stephen Worthley, Peter Psaltis] Despite current treatment options, cardiac failure is associated with significant morbidity and mortality highlighting a compelling clinical need for novel therapeutic approaches. Based on promising pre-clinical data, stem cell therapy has been suggested as a possible therapeutic strategy. Of the candidate cell types evaluated, mesenchymal stromal/stem cells (MSCs) have been widely evaluated due to their ease of isolation and ex vivo expansion, potential allogeneic utility and capacity to promote neo-angiogenesis and endogenous cardiac repair. However, the clinical application of MSCs for mainstream cardiovascular use is currently hindered by several important limitations, including suboptimal retention and engraftment and restricted capacity for bona fide cardiomyocyte regeneration. Consequently, this has prompted intense efforts to advance the therapeutic properties of MSCs for cardiovascular disease. In this review, we consider the scope of benefit from traditional plastic adherence-isolated MSCs and the lessons learned from their conventional use in preclinical and clinical studies. Focus is then given to the evolving strategies aimed at optimizing MSC therapy, including discussion of cell-targeted techniques that encompass the preparation, pre-conditioning and manipulation of these cells ex vivo, methods to improve their delivery to the heart and innovative substrate-directed strategies to support their interaction with the host myocardium. Springer Science+Business Media, LLC, 2012 Cardiomyopathy nationallicence Ischemic heart disease nationallicence Limitations nationallicence Mesenchymal stem cells nationallicence Mesenchymal precursor cells nationallicence Myocardial infarction nationallicence Optimization nationallicence Paracrine nationallicence Pre-conditioning nationallicence Tissue engineering nationallicence Richardson James Cardiovascular Research Centre, Royal Adelaide Hospital and Department of Medicine, University of Adelaide, Adelaide, South Australia, Australia aut Nelson Adam Cardiovascular Research Centre, Royal Adelaide Hospital and Department of Medicine, University of Adelaide, Adelaide, South Australia, Australia aut Zannettino Andrew Department of Haematology, SA Pathology and Centre for Stem Cell Research, Robinson Institute, Discipline of Medicine, University of Adelaide, Adelaide, South Australia, Australia aut Gronthos Stan Department of Haematology, SA Pathology and Centre for Stem Cell Research, Robinson Institute, Discipline of Medicine, University of Adelaide, Adelaide, South Australia, Australia aut Worthley Stephen Cardiovascular Research Centre, Royal Adelaide Hospital and Department of Medicine, University of Adelaide, Adelaide, South Australia, Australia aut Psaltis Peter Cardiovascular Research Centre, Royal Adelaide Hospital and Department of Medicine, University of Adelaide, Adelaide, South Australia, Australia aut Stem Cell Reviews and Reports Humana Press Inc 9/3(2013-06-01), 281-302 1550-8943 9:3<281 2013 9 12015 https://doi.org/10.1007/s12015-012-9366-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-012-9366-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Richardson James Cardiovascular Research Centre, Royal Adelaide Hospital and Department of Medicine, University of Adelaide, Adelaide, South Australia, Australia aut NATIONALLICENCE 700 1- Nelson Adam Cardiovascular Research Centre, Royal Adelaide Hospital and Department of Medicine, University of Adelaide, Adelaide, South Australia, Australia aut NATIONALLICENCE 700 1- Zannettino Andrew Department of Haematology, SA Pathology and Centre for Stem Cell Research, Robinson Institute, Discipline of Medicine, University of Adelaide, Adelaide, South Australia, Australia aut NATIONALLICENCE 700 1- Gronthos Stan Department of Haematology, SA Pathology and Centre for Stem Cell Research, Robinson Institute, Discipline of Medicine, University of Adelaide, Adelaide, South Australia, Australia aut NATIONALLICENCE 700 1- Worthley Stephen Cardiovascular Research Centre, Royal Adelaide Hospital and Department of Medicine, University of Adelaide, Adelaide, South Australia, Australia aut NATIONALLICENCE 700 1- Psaltis Peter Cardiovascular Research Centre, Royal Adelaide Hospital and Department of Medicine, University of Adelaide, Adelaide, South Australia, Australia aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Humana Press Inc 9/3(2013-06-01), 281-302 1550-8943 9:3<281 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer