naa a22 4500 510739229 CHVBK 20180411083022.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s12015-012-9367-6 doi (NATIONALLICENCE)springer-10.1007/s12015-012-9367-6 Comparison of Cardiac Stem Cells and Mesenchymal Stem Cells Transplantation on the Cardiac Electrophysiology in Rats with Myocardial Infarction [Elektronische Daten] [Shao-Xin Zheng, Yin-Lun Weng, Chang-Qing Zhou, Zhu-Zhi Wen, Hui Huang, Wei Wu, Jing-Feng Wang, Tong Wang] Introduction: Whether transplanted cardiac stem cells (CSCs) and mesenchymal stem cells (MSCs) improved ventricular fibrillation threshold (VFT) similarly is still unclear. We sought to compare the effects of the CSC and MSC transplantation on the electrophysiological characteristics and VFT in rats with myocardial infarction (MI). Methods: MI was induced in 30 male Sprague-Dawley rats. Two weeks later, animals were randomized to receive 5 × 106 CSCs labeled with PKH26 in PBS or 5 × 106 MSCs labeled with PKH26 in phosphate buffer solution(PBS) or PBS alone injection into the infarcted anterior ventricular free wall. Six weeks after the injection, electrophysiological characteristics and VFT were measured. Labeled CSCs and MSCs were observed in 5μm cryostat sections from each heart. Results: Malignant ventricular arrhythmias were significantly (P = 0.0055) less inducible in the CSC group than the MSC group. The VFTs were improved in the CSC group compared with the MSC group. Labeled CSCs and MSCs were identified in the infarct zone and infarct marginal zone. Labeled CSCs expressed Connexin-43, von Willebrand factor, α-smooth muscle actin and α-sarcomeric actin,while the Labeled MSCs expressed von Willebrand factor, α-smooth muscle actin and α-sarcomeric actin in vivo. Conclusions: After 6weeks of cell transplantation, CSCs are superior to MSCs in modulating the electrophysiological abnormality and improving the VFT in rats with MI. CSCs and MSCs express markers that suggest muscle, endothelium and vascular smooth muscle phenotypes in vivo, but MSCs rarely express Connexin-43. Springer Science+Business Media, LLC, 2012 Cardiac stem cells nationallicence Mesenchymal stem cells nationallicence Electrophysiological characteristics nationallicence Myocardial infarction nationallicence Ventricular fibrillation threshold nationallicence ART : Activation recovery time nationallicence ARTd : ART dispersion nationallicence ARTc : Correct ART nationallicence ARTcd : ARTc dispersion nationallicence Cx43 : Connexin-43 nationallicence CSCs : Cardiac stem cells nationallicence MSCs : Mesenchymal stem cells nationallicence MI : Myocardial infarction nationallicence PBS : Phosphate buffer solution nationallicence VT : Ventricular tachycardia nationallicence VFT : Ventricular fibrillation threshold nationallicence VF : Ventricular fibrillation nationallicence Zheng Shao-Xin Cardiovascular Medicine, The Sun Yat-sen Memorial Hospital of Sun Yat-sen University. Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, 107 Yanjiang Xi Road, 510120, Guangzhou, China aut Weng Yin-Lun Emergency Medicine, The Sun Yat-sen Memorial Hospital of Sun Yat-sen University, 107 Yanjiang Xi Road, 510120, Guangzhou, China aut Zhou Chang-Qing Emergency Medicine, The Sun Yat-sen Memorial Hospital of Sun Yat-sen University, 107 Yanjiang Xi Road, 510120, Guangzhou, China aut Wen Zhu-Zhi Cardiovascular Medicine, The Sun Yat-sen Memorial Hospital of Sun Yat-sen University. Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, 107 Yanjiang Xi Road, 510120, Guangzhou, China aut Huang Hui Cardiovascular Medicine, The Sun Yat-sen Memorial Hospital of Sun Yat-sen University. Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, 107 Yanjiang Xi Road, 510120, Guangzhou, China aut Wu Wei Cardiovascular Medicine, The Sun Yat-sen Memorial Hospital of Sun Yat-sen University. Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, 107 Yanjiang Xi Road, 510120, Guangzhou, China aut Wang Jing-Feng Cardiovascular Medicine, The Sun Yat-sen Memorial Hospital of Sun Yat-sen University. Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, 107 Yanjiang Xi Road, 510120, Guangzhou, China aut Wang Tong Cardiovascular & Emergency Medicine, The Sun Yat-sen Memorial Hospital of Sun Yat-sen University. Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, 107 Yanjiang Xi Road, 510120, Guangzhou, China aut Stem Cell Reviews and Reports Humana Press Inc 9/3(2013-06-01), 339-349 1550-8943 9:3<339 2013 9 12015 https://doi.org/10.1007/s12015-012-9367-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-012-9367-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zheng Shao-Xin Cardiovascular Medicine, The Sun Yat-sen Memorial Hospital of Sun Yat-sen University. Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, 107 Yanjiang Xi Road, 510120, Guangzhou, China aut NATIONALLICENCE 700 1- Weng Yin-Lun Emergency Medicine, The Sun Yat-sen Memorial Hospital of Sun Yat-sen University, 107 Yanjiang Xi Road, 510120, Guangzhou, China aut NATIONALLICENCE 700 1- Zhou Chang-Qing Emergency Medicine, The Sun Yat-sen Memorial Hospital of Sun Yat-sen University, 107 Yanjiang Xi Road, 510120, Guangzhou, China aut NATIONALLICENCE 700 1- Wen Zhu-Zhi Cardiovascular Medicine, The Sun Yat-sen Memorial Hospital of Sun Yat-sen University. Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, 107 Yanjiang Xi Road, 510120, Guangzhou, China aut NATIONALLICENCE 700 1- Huang Hui Cardiovascular Medicine, The Sun Yat-sen Memorial Hospital of Sun Yat-sen University. Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, 107 Yanjiang Xi Road, 510120, Guangzhou, China aut NATIONALLICENCE 700 1- Wu Wei Cardiovascular Medicine, The Sun Yat-sen Memorial Hospital of Sun Yat-sen University. Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, 107 Yanjiang Xi Road, 510120, Guangzhou, China aut NATIONALLICENCE 700 1- Wang Jing-Feng Cardiovascular Medicine, The Sun Yat-sen Memorial Hospital of Sun Yat-sen University. Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, 107 Yanjiang Xi Road, 510120, Guangzhou, China aut NATIONALLICENCE 700 1- Wang Tong Cardiovascular & Emergency Medicine, The Sun Yat-sen Memorial Hospital of Sun Yat-sen University. Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, 107 Yanjiang Xi Road, 510120, Guangzhou, China aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Humana Press Inc 9/3(2013-06-01), 339-349 1550-8943 9:3<339 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer