naa a22 4500 510739253 CHVBK 20180411083022.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s12015-012-9375-6 doi (NATIONALLICENCE)springer-10.1007/s12015-012-9375-6 Mesenchymal Stem Cells for Cardiac Therapy: Practical Challenges and Potential Mechanisms [Elektronische Daten] [Timothy Cashman, Valerie Gouon-Evans, Kevin Costa] Cell based treatments for myocardial infarction have demonstrated efficacy in the laboratory and in phase I clinical trials, but the understanding of such therapies remains incomplete. Mesenchymal stem cells (MSCs) are classically defined as maintaining the ability to generate mesenchyme-derived cell types, namely adipocytes, chondrocytes and osteocytes. Recent evidence suggests these cells may in fact harbor much greater potency than originally realized, as several groups have found that MSCs can form cardiac lineage cells in vitro. Additionally, experimental coculture of MSCs with cardiomyocytes appears to improve contractile function of the latter. Bolstered by such findings, several clinical trials have begun to test MSC transplantation for improving post-infarct cardiac function in human patients. The results of these trials have been mixed, underscoring the need to develop a deeper understanding of the underlying stem cell biology. To help synthesize the breadth of studies on the topic, this paper discusses current challenges in the field of MSC cellular therapies for cardiac repair, including methods of cell delivery and the identification of molecular markers that accurately specify the therapeutically relevant mesenchymal cell types. The various possible mechanisms of MSC mediated cardiac improvement, including somatic reprogramming, transdifferentiation, paracrine signaling, and direct electrophysiological coupling are also reviewed. Finally, we consider the traditional cell culture microenvironment, and the promise of cardiac tissue engineering to provide biomimetic in vitro model systems to more faithfully investigate MSC biology, helping to safely and effectively translate exciting discoveries in the laboratory to meaningful therapies in the clinic. Springer Science+Business Media, LLC, 2012 Marrow stromal cell nationallicence Cardiac repair nationallicence Cellular therapy nationallicence Molecular markers nationallicence Reprogramming nationallicence Differentiation nationallicence Paracrine signaling nationallicence Tissue engineering nationallicence Cashman Timothy Cardiovascular Cell and Tissue Engineering Laboratory, Cardiovascular Research Center, Mount Sinai School of Medicine, One Gustave L. Levy Place, Box 1030, 10029, New York, NY, USA aut Gouon-Evans Valerie Black Family Stem Cell Institute, Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, One Gustave L. Levy Place, Box 1020, 10029, New York, NY, USA aut Costa Kevin Cardiovascular Cell and Tissue Engineering Laboratory, Cardiovascular Research Center, Mount Sinai School of Medicine, One Gustave L. Levy Place, Box 1030, 10029, New York, NY, USA aut Stem Cell Reviews and Reports Humana Press Inc 9/3(2013-06-01), 254-265 1550-8943 9:3<254 2013 9 12015 https://doi.org/10.1007/s12015-012-9375-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-012-9375-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cashman Timothy Cardiovascular Cell and Tissue Engineering Laboratory, Cardiovascular Research Center, Mount Sinai School of Medicine, One Gustave L. Levy Place, Box 1030, 10029, New York, NY, USA aut NATIONALLICENCE 700 1- Gouon-Evans Valerie Black Family Stem Cell Institute, Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, One Gustave L. Levy Place, Box 1020, 10029, New York, NY, USA aut NATIONALLICENCE 700 1- Costa Kevin Cardiovascular Cell and Tissue Engineering Laboratory, Cardiovascular Research Center, Mount Sinai School of Medicine, One Gustave L. Levy Place, Box 1030, 10029, New York, NY, USA aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Humana Press Inc 9/3(2013-06-01), 254-265 1550-8943 9:3<254 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer