naa a22 4500 51073927X CHVBK 20180411083022.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s12015-012-9420-5 doi (NATIONALLICENCE)springer-10.1007/s12015-012-9420-5 Induced Pluripotent Stem Cells from Human Hair Follicle Mesenchymal Stem Cells [Elektronische Daten] [Yimei Wang, Jinyu Liu, Xiaohua Tan, Gaofeng Li, Yunhe Gao, Xuejuan Liu, Lihong Zhang, Yulin Li] Reprogramming of somatic cells into inducible pluripotent stem cells (iPSCs) provides an alternative to using embryonic stem cells (ESCs). Mesenchymal stem cells derived from human hair follicles (hHF-MSCs) are easily accessible, reproducible by direct plucking of human hairs. Whether these hHF-MSCs can be reprogrammed has not been previously reported. Here we report the generation of iPSCs from hHF-MSCs obtained by plucking several hairs. hHF-MSCs were isolated from hair follicle tissues and their mesenchymal nature confirmed by detecting cell surface antigens and multilineage differentiation potential towards adipocytes and osteoblasts. They were then reprogrammed into iPSCs by lentiviral transduction with Oct4, Sox2, c-Myc and Klf4. hHF-MSC-derived iPSCs appeared indistinguishable from human embryonic stem cells (hESCs) in colony morphology, expression of alkaline phosphotase, and expression of specific hESCs surface markers, SSEA-3, SSEA-4, Tra-1-60, Tra-1-81, Nanog, Oct4, E-Cadherin and endogenous pluripotent genes. When injected into immunocompromised mice, hHF-MSC-derived iPSCs formed teratomas containing representatives of all three germ layers. This is the first study to report reprogramming of hHF-MSCs into iPSCs. The Author(s), 2012 Induced pluripotent stem cells nationallicence Mesenchymal stem cells nationallicence Human hair follicle nationallicence Reprogrammed nationallicence iPSCs : Induced pluripotent stem cells nationallicence ESCs : Embryonic stem cells nationallicence hESCs : Human embryonic stem cells nationallicence MSCs : Mesenchymal stem cells nationallicence hHF-MSCs : Human hair follicle mesenchymal stem cells nationallicence MEFs : Mouse embryonic fibroblasts nationallicence Wang Yimei The Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, People's Republic of China aut Liu Jinyu The Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, People's Republic of China aut Tan Xiaohua The Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, People's Republic of China aut Li Gaofeng Department of Spinal Surgery, Jilin Provincial People's Hospital, Changchun, People's Republic of China aut Gao Yunhe The Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, People's Republic of China aut Liu Xuejuan The Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, People's Republic of China aut Zhang Lihong The Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, People's Republic of China aut Li Yulin The Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, People's Republic of China aut Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/4(2013-08-01), 451-460 1550-8943 9:4<451 2013 9 12015 https://doi.org/10.1007/s12015-012-9420-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-012-9420-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Yimei The Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Liu Jinyu The Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Tan Xiaohua The Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Li Gaofeng Department of Spinal Surgery, Jilin Provincial People's Hospital, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Gao Yunhe The Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Liu Xuejuan The Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Lihong The Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Li Yulin The Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/4(2013-08-01), 451-460 1550-8943 9:4<451 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer